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KK3009

Roche

KAPA Taq EXtra PCR Kit

Sinonimo/i:

PCR, Taq

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About This Item

Codice UNSPSC:
41106300
NACRES:
NA.55

impiego

sufficient for 500 reactions

Livello qualitativo

100

Caratteristiche

High Fidelity PCR
Long Range PCR
dNTPs included
hotstart: no

Confezionamento

pkg of 250 U

Produttore/marchio commerciale

Roche

tecniche

PCR: suitable

input

purified DNA

Temperatura di conservazione

−20°C

Applicazioni

The KAPA Taq EXtra PCR system is ideally suited for:
  • PCR of short- and medium-length targets (<5 kb)
  • Long-range PCR (5–15 kb)
  • PCR with limiting amounts of template DNA.

Azioni biochim/fisiol

The KAPA Taq EXtra PCR system is a blend of KAPA Taq DNA polymerase and a modified archaeal (Type B) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support robust, long range, and sensitive PCR. Both enzymes possess 5′→3′ polymerase activity, but only KAPA Taq DNA polymerase possesses 5′→3′ exonuclease activity and only the Type B DNA polymerase possesses 3′→5′ exonuclease (proofreading) activity. Reaction products generated with KAPA Taq EXtra are polyA-tailed, and can be cloned into TA-cloning vectors.

Caratteristiche e vantaggi

  • KAPA Taq EXtra is a blend of KAPA Taq and a proofreading DNA polymerase.
  • Suitable for short-, medium- and long-range PCR, and PCR with low amounts of template DNA.
  • Fidelity is 2-4 times better than Taq
  • For short amplicons, replace Taq DNA polymerase for improvements in yield and/or fidelity.
  • Amplify mid- to long-range targets with high yield and sensitivity.
  • KAPA Taq EXtra Buffer (Mg-free) is designed for long-range, sensitive PCR. MgCl2 is supplied separately for optimization.
  • The fidelity of KAPA Taq EXtra is 2-4 fold higher than that of KAPA Taq DNA Polymerase, but is lower than the fidelity of pure proofreading polymerases, such as KAPA HiFi.

Qualità

Each batch of KAPA Taq EXtra DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq EXtra PCR kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.

Nota sulla preparazione

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage.

Altre note

For Research Use Only. Not for use in diagnostic procedures.

Solo come componenti del kit

N° Catalogo
Descrizione
  • KAPA Taq EXtra DNA Polymerase 5 U/µL
  • KAPA Taq EXtra Buffer (5X)
  • KAPA dNTP Mix 10 mM each
  • MgCl2 25 mM

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Gene polymorphism biomarkers identify individual susceptibility to environmental and occupational hazards. The conventional approach considers polymerase chain reaction (PCR) followed by restriction fragment length polymorphism analysis (RFLP), a reliable but expensive and time-consuming two-step procedure. Therefore we evaluated the simpler
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Cigarette smoking is influenced by nicotine's effects on dopaminergic activity, which appear to be moderated by genetic variation, particularly a variable number tandem repeat (VNTR, 48 bp) polymorphism in the third exon of the dopamine receptor gene (DRD4). Smokers with

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