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11299964001

Roche

5-Bromo-2′-deoxy-uridine Labeling and Detection Kit II

sufficient for ≤100 tests, storage temp.:-20°C

Sinonimo/i:

5-BrdU, 5-Bromo-2-deoxyuridine

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
41116133

impiego

sufficient for ≤100 tests

Produttore/marchio commerciale

Roche

Temperatura di conservazione

−20°C

Descrizione generale

Cell proliferation may be studied by monitoring the incorporation of a radioisotope, [ 3H]-thymidine, into cellular DNA, followed by autoradiography.
Alternatively, 5-bromo-2′-deoxy-uridine (BrdU) may be used instead of thymidine. Cells that have incorporated BrdU into DNA are easily detected using a monoclonal antibody against BrdU and an enzyme- or fluorochrome-conjugated second antibody.
Immunohistocytochemical assay for the detection of 5-bromo-2′-deoxy-uridine (BrdU) incorporated into cellular DNA.

Specificità

Anti-BrdU monoclonal antibody specifically binds to 5-bromo-2′-deoxy-uridine, and shows cross-reactivity with 5-iodo-2′-deoxy-uridine (10%). Anti-BrdU shows no cross-reactivity with 5-fluoro-2′-deoxy-uridine or any endogenous cellular component, such as thymidine or uridine.

Applicazioni

5-Bromo-2′-deoxy-uridine Labeling and Detection Kit II has been used in:
  • labeling of tooth roots for histology[1]
  • immunostaining of mice frontal sections[2]
  • immunofluorescence imaging of hepatocellular carcinoma sections[3]

The kit is used for the detection of BrdU incorporated into cellular DNA by immunohistocytochemistry.[4][5][6]

Caratteristiche e vantaggi

  • Safe: No radioisotopes are used.
  • Easy to perform: Follows a standard immunohistochemistry protocol.
  • Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU.
  • Flexible: Allows double-labeling protocols.

Confezionamento

1 kit containing 7 components.

Principio

Samples prelabeled with BrdU are fixed with ethanol, then incubated with a monoclonal antibody to BrdU, which contains an optimized mixture of nucleases. These nucleases generate single-stranded DNA fragments that allow binding of the antibody to BrdU. Next, an alkaline phosphatase (AP)-labeled antibody to mouse immunoglobulin is added, then bound to the anti-BrdU antibody. The sample is then incubated with the AP substrate and NBT/ BCIP, which is metabolized to form a colored reaction product. The sample is evaluated using a phase-contrast microscope.

Nota sulla preparazione

Working concentration: Working concentration of the labeling reagent corresponds to the WC of the In Situ Cell Proliferation Kits.
Sample material:
Cell culture:
adherent cells, suspension cells, organ or explant cultures. Frozen or paraffin-embedded tissue sections (after in vivo labeling).

Altre note

For life science research only. Not for use in diagnostic procedures.

Solo come componenti del kit

N° Catalogo
Descrizione

  • BrdU Labeling Reagent 1,000x concentrated

  • Washing Buffer concentrate 10x concentrated

  • Incubation Buffer

  • Anti-BrdU antibody, contains nucleases for DNA denaturation

  • Anti-mouse Ig-alkaline Phosphatase antibody

  • NBT

  • BCIP

Avvertenze

Danger

Classi di pericolo

Acute Tox. 4 - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 Dermal - Muta. 1B - Repr. 1B - Skin Sens. 1

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

136.4 °F

Punto d’infiammabilità (°C)

58 °C


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Articoli

Saggi basati su cellule per valutare la proliferazione cellulare (BrdU, MTT, WST1), la vitalità cellulare e la citotossicità nel campo della ricerca oncologica, neuroscientifica e delle cellule staminali.

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.