605190-M

Thrombin, Human Plasma

• Sinonimo/i: Thrombin, Human Plasma
• Numero CAS: 9002-04-4
• Numero MDL: MFCD00007020
• Codice UNSPSC: 12352202
• NACRES: NA.51

Proprietà

• Origine biologica: human plasma
• Livello qualitativo: 100
• Descrizione: Merck USA index - 14, 9383
• Stato: lyophilized
• Attività specifica: ≥1000 NIH units/mg protein
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Solubilità: water: 1 mg/mL; aqueous buffer: soluble
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Thrombin, a sodium-activated type II enzyme, comprises two anion binding exosites, ABE-I and ABE-II.[1] This serine protease enzyme is synthesized from zymogen prothrombin (factor II) in the liver.[1][2]

Applicazioni

Thrombin, Human Plasma has been used:

• as a component of endothelial growth medium (EGM) media for the transplantation and reisolation of Kaposi′s sarcoma-associated herpesvirus-human endothelial cell line (KSHV-HuARLT) cells from mice[3]
• for the fabrication of fibrin gels[4]
• as a component of EGM media for viral copy number analysis of KSHV-HuARLT cells and matrigel implant[5]

Azioni biochim/fisiol

Thrombin cleaves and converts fibrinogen into fibrin. It then activates factors V, VIII, XI, and XIII. Thrombin stimulates platelet activation and stabilizes the fibrin polymers.[6][7] It elicits a vital role in the last stages of the blood coagulation cascade.[1]

Attenzione

Toxicity: Harmful (C)

Definizione di unità

One unit is determined by comparison with a standard curve prepared using the Bureau of Biologics standard thrombin.

Stato fisico

Lyophilized from 200 mM NaCl, 50 mM citrate buffer, 0.1% PEG-8000, pH 6.5. Contains BSA as a stabilizer.

Nota sulla preparazione

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Ricostituzione

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 2 months at -70°C.

Risultati analitici

Complete activation from homogeneous prothrombin by SDS-PAGE

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H315,H319,H334,H335
• Consigli di prudenza: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Classi di pericolo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable