The maximum size of cell clumps that can pass through the Scepter 3.0 is determined by the aperture limits, which are 40um and 60um for sensors 40 and 60, respectively. Unlike the Scepter 2.0, the Scepter 3.0 does not use a screen to break up cell clump aggregates; instead, it employs a set of pillars to achieve this function. In contrast, the Scepter 2.0 utilizes a 35um Nylon filter to break up cell clump aggregates.
PHCC360KIT
Contacellule automatico palmare Scepter 3.0
Rapid cell counts in a handheld easy to use format, includes pkg of 60 μm Scepter 3.0 Cell Counter Sensors
Sinonimo/i:
Cell counting instrument
Scegli un formato
Scegli un formato
About This Item
Prodotti consigliati
Produttore/marchio commerciale
Scepter
tecniche
cell counting: suitable
Dimensione particelle
6-36 μm
Compatibilità
for use with cells sized 4.0–25.0 μm
Condizioni di spedizione
ambient
Descrizione generale
- Concentrazione delle cellule
- Dimensioni medie delle cellule
- Volume medio delle cellule
- Istogramma della distribuzione di dimensioni o volume
Il contacellule Scepter 3.0 è compatibile con i sensori per contacellule Scepter 3.0 da 40 μM e 60 μM. Questo kit (PHCC360KIT) comprende una confezione gratuita da 50 sensori da 60μM.
Componenti
- 1 Contacellule automatico palmare Scepter 3.0
- 1 Fiala di sferette di prova
- 1 Stazione di ricarica
- 50 Sensori (60 μM)
Altre note
Accessorio
Codice della classe di stoccaggio
10-13 - German Storage Class 10 to 13
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Articoli
Data for validated human cell lines using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.
The Scepter™ 3.0 Cell Counter, when used in conjunction with a 40 μm aperture sensor, can accurately count and precisely size a wide range of cell types and particles. This application note demonstrates two examples of experiments using the instrument’s sensitive size-discriminating capacity for rapid qualitative assessment of individual cell populations.
Learn how Sterivex™ pressure-driven sterile filters can be used for large-volume sample processing in environmental research and testing.
Protocolli
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Contenuto correlato
Velocizzate l’acquisizione e l’analisi dei dati nelle misurazioni della resistenza elettrica transepiteliale (TEER) grazie a migliorie vantaggiose per la semplicità d’impiego, tra cui l’interfaccia touchscreen dall’uso intuitivo, la sonda autoportante in-well e l’acquisizione automatica dei dati.
Monitor your cell cultures with ease with the Millicell® DCI Digital Cell Imager. From confluency to cell count and morphology, the Millicell® DCI automates cell culture measurements for quicker, more consistent culturing.
Scepter™ Cell Counter is a portable device which brings consistency in cell counting right to the culture hood in less than 30 seconds.
Il contacellule Scepter™ è un apparecchio portatile che consente di realizzare conteggi cellulari accurati e riproducibili direttamente nella cappa di coltura in meno di 30 secondi.
-
What is the maximum size of cell clumps that can pass through the Scepter 3.0? Also, what size screen is used to break up the cell clump aggregates in both Scepter 2.0 and 3.0?
1 answer-
Helpful?
-
-
Is this appropriate for size 15-23 μm cell? Does the Kit include also the charger? How many 60/40 μm sensors are included Which sensors can be used with this instrument? Which sensors are appropriate for cells size 15-23 μm and what is their price?
1 answer-
Please see the answers to the following questions below:
1. Is this appropriate for size 15-23 μm cells?
Yes, the Scepter 3.0 is suitable. As listed on the product detail page in the DESCRIPTION section for use with cells sized 4.0–25.0 μm.2. Does the Kit include the charger?
This product does include 60μM sensors and a charging station.3. How many 60/40 μm sensors are included
As mentioned on the product detail page in the DESCRIPTION section under 'Components', it includes 50 Sensors.4. Which sensors can be used with this instrument?
This product is only compatible with Scepter 3.0 Cell Counter Sensors shown in the Related Products on this page. Please see the link below to review additional information regarding the Scepter 3.0:
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-counting-and-health-analysis/scepter-cell-counter5. Which sensors are appropriate for cells size 15-23 μm and what is their price?
See question 1, this product is suitable for the cell size of 15-23 μm.Regarding pricing, Please navigate to the link https://www.sigmaaldrich.com/support/customer-support, and click on "Products Pricing & Availability" under the Products Section with all the required information so that a member of the Customer Service team can reach out to assist further. Thank you.
Helpful?
-
-
Does it require Trypan blue or another dye? Does it distinguish between live and dead cells?
1 answer-
The Scepter is a particle counter, but it will not differentiate live from dead cells. The device can be used to gauge cell health on the basis of cell size. For example, in cell types where apoptotic cells are smaller in size compared to non-apoptotic cells. This shift in size can be quantified using the device option "Edit Histogram" to either gate around this population or exclude the population from the count.
Please see the following manual below for more information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/799/484/00110230w-rev1020-ms.pdfHelpful?
-
Active Filters
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.