MAB3408
Anti-Tubulin Antibody, beta, clone KMX-1
clone KMX-1, Chemicon®, from mouse
Sinonimo/i:
Tubulin beta-III, tubulin, beta 3, tubulin, beta 4
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
KMX-1, monoclonal
Reattività contro le specie
human, rat, mouse
Reattività contro le specie (prevista in base all’omologia)
all
Produttore/marchio commerciale
Chemicon®
tecniche
immunocytochemistry: suitable
western blot: suitable
Isotipo
IgG2b
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... TUBB3(10381)
Descrizione generale
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues.
Specificità
This antibody reacts specifically with β-tubulin and shows a strong cross-reactivity to different species. This antibody allows staining of microtubuli using indirect immunofluorescence showing a low background only.
Immunogeno
Tubulin purified from polycephalum myxamoebae
Applicazioni
Immunocytochemistry:
A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry.
Western blot:
0.05-0.5 μg/mL
Optimal working dilutions must be determined by end user.
A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry.
Western blot:
0.05-0.5 μg/mL
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-Tubulin Antibody, beta, clone KMX-1 is validated for use in IC, WB for the detection of Tubulin.
Qualità
Routinely evaluated by Western Blot on A431 lysates.
Western blot:
1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates.
Western blot:
1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates.
Descrizione del bersaglio
50 kDa
Linkage
Replaces: 04-1049
Stato fisico
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2b liquid in buffer containing 0.02M phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Stoccaggio e stabilità
Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
Risultati analitici
Control
HeLa Cell lysate
A431 Cell lysate
MCF7 Cell lysate
HeLa Cell lysate
A431 Cell lysate
MCF7 Cell lysate
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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