ABE2902

Anti-Cdc45

from rabbit

• Sinonimo/i: DNA replication protein Cdc45
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Reattività contro le specie: mouse, hamster, human
• tecniche: western blot: suitable
• Isotipo: IgG
• N° accesso NCBI: NP_001233719
• N° accesso UniProt: Q4U3P7
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: hamster ... Cdc45(100689362) ; human ... CDC45(8318) ; mouse ... Cdc45(12544)

Descrizione

Descrizione generale

DNA replication protein Cdc45 (UniProt: Q4U3P7; also known as Cdc45) is encoded by the CDC45 gene (Gene ID: 100689362) in hamster. Cdc45 is a replication initiation protein that plays an essential role in the establishment and progression of the replication fork. It is required for formation of pre-initiation complexes at the G1/S transition and for activation of replication origins. Cdc45 associates with the heterohexameric Mcm2-7 helicase and the GINS complex (go-ichi-nii-san, Japanese for 5-1-2-3, for the subunits Sld5, Psf1, Psf2 and Pfs3) to form the CMG (Cdc45-Mcm2-7-GINS) complex. CMG represents the eukaryotic replicative DNA helicase that unwinds origins of replication and drives the replication fork. Cdc45 is also reported to interact with several other replication proteins, including DNA helicase B (DHB), Mcm10, Orc2, TopPB1, and DNA polymerases alpha, delta and epsilon. Expression of Cdc45 is associated with the proliferative status of the cell or tissue. Terminally differentiated and senescent cells do not display Cdc45 activity whereas highly proliferative cells express high levels of Cdc45.

Specificità

This rabbit polyclonal antibody detects Cdc45 in Human and Murine cells.

Immunogeno

His-tagged full length hamster recombinant Cdc45.

Applicazioni

Anti-Cdc45, Cat. No. ABE2902, is a highly specific rabbit polyclonal antibody that targets DNA replication protein Cdc45 and has been tested for use in Western Blotting.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Cdc45 in 10 µg of HeLa cell lysate.

Western Blotting Analysis: A representative lot detected Cdc45 in asynchronous, go synchronized and G1 released CHO cells, immunoprecipitated CDC45 from CHO cells, HeLa and NIH3T3 (Wong, P.G., et. al. (2011). PLoS One. 6(3):e17533).

Qualità

Evaluated by Western Blotting in CHO cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Cdc45 in 10 µg of CHO cell lysate.

Descrizione del bersaglio

~65 kDa observed; 65.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Altre note

Concentration: Please refer to lot specific datasheet.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable