ABE1867
Anti-MLL4 Antibody
from rabbit, purified by affinity chromatography
Sinonimo/i:
Histone-lysine N-methyltransferase 2D, ALL1-related protein, Lysine N-methyltransferase 2D, Myeloid/lymphoid or mixed-lineage leukemia protein 2
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Purificato mediante
affinity chromatography
Reattività contro le specie
human, mouse
tecniche
ChIP: suitable (ChIP-seq)
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
ambient
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... KMT2D(8085)
Descrizione generale
Histone-lysine N-methyltransferase 2D (EC 2.1.1.43; UniProt O14686; also known as MLL4, ALL1-related protein, Lysine N-methyltransferase 2D) is encoded by the KMT2D (also known as MLL4, ALR, KABUK1, MLL2) gene (Gene ID: 8085) in human. MLL4 methylates Lys-4 of histone H3 to form H3K4 mono- and di-methylation (H3K4me1/2), which represents a specific tag for transcriptional enhancers. MLL4 is also a component of the MLL3/4 protein complex that plays an important role during cell differentiation and animal development. MLL4 is localized to the nucleus and is expressed in most embryonic and adult tissues. Defects in MLL4 (often known as KMT2D in the literature) have been linked to Kabuki syndrome and congenital heart diseases. Inactivating mutations in MLL4/MLL2/KMT2D gene have been linked to many types of cancers, including medulloblastoma, non-Hodgkin lymphoma, diffuse large B-cell lymphoma, prostate cancer, breast cancer, hepatocellular carcinoma, ovarian cancer, lung, bladder, head & neck cancers, and pancreatic cancer. (Ref.: Lee, J.E., et al. (2013). Elife. 2:e01503; Lawrence, M.S., et al., (2014). Nature. 505(7484):495-501).
Specificità
This polyclonal antibody targets the region between the first and third coiled coil of MLL4 sequence.
Immunogeno
Epitope: unknown
His-tagged recombinant human MLL4 internal fragment from the region between the first and third coiled coil.
Applicazioni
Anti-MLL4, Cat. No. ABE1867, is a highly specific rabbit polyclonal antibody that targets MLL4 (also known as KMT2D) and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq, Immunoprecipitation, and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Western Blotting Analysis: A 1:500 dilution of the unpurified antiserum detected the expression of Myc-tagged human MLL4 a.a. 2916-3785, but not a.a. 3662-4625, fragment in 100 µg whole cell lysate from respective transfectants (Courtesy of Dr Lee from NIH).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected MLL4 (MLL2) occupancy on the PPAR 1, but not the PPAR 2, proximal promoter in MEFs, while MLL4 enrichment was found on PPARγ1, PPARγ2, and C/EBP promoters in differentiating murine brown preadipocytes (Cho, Y.W., et al. (2009). Cell Metab. 10(1):27-39).
ChIP-Sequencing (ChIP-seq) Analysis: A representative lot detected MLL4 MLL4 genomic enrichment sites in a cell type- and differentiation stage-specific manner (Lee, J.E., et al. (2013). Elife. 2:e01503).
Immunoprecipitation Analysis: A representative lot immunoprecipitated MLL3/MLL4 complex components (UTX, PTIP, RbBP5 and PA1) and C/EBPβ, but not Menin, from mouse brown preadipocyte nuclear extracts prepared at day 2 of adipogenesis (Lee, J.E., et al. (2013). Elife. 2:e01503).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected MLL4 (MLL2) occupancy on the PPAR 1, but not the PPAR 2, proximal promoter in MEFs, while MLL4 enrichment was found on PPARγ1, PPARγ2, and C/EBP promoters in differentiating murine brown preadipocytes (Cho, Y.W., et al. (2009). Cell Metab. 10(1):27-39).
ChIP-Sequencing (ChIP-seq) Analysis: A representative lot detected MLL4 MLL4 genomic enrichment sites in a cell type- and differentiation stage-specific manner (Lee, J.E., et al. (2013). Elife. 2:e01503).
Immunoprecipitation Analysis: A representative lot immunoprecipitated MLL3/MLL4 complex components (UTX, PTIP, RbBP5 and PA1) and C/EBPβ, but not Menin, from mouse brown preadipocyte nuclear extracts prepared at day 2 of adipogenesis (Lee, J.E., et al. (2013). Elife. 2:e01503).
Qualità
Evaluated by Western Blotting of recombinant MLL4 fragment.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected 50 ng of recombinant human MLL4 fragment.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected 50 ng of recombinant human MLL4 fragment.
Descrizione del bersaglio
593.4/593.7 kDa (human isoform 1/2) and 600.2 kDa (mouse) calculated.
Stato fisico
Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Altre note
Concentration: Please refer to lot specific datasheet.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Certificati d'analisi (COA)
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