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ABE101-S

Sigma-Aldrich

Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size

from rabbit, purified by affinity chromatography

Sinonimo/i:

H3K9me1, Histone H3 (monomethyl K9), H3 histone family, member T, Histone 3, H3, Histone cluster 3, H3

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Purificato mediante

affinity chromatography

Reattività contro le specie

human

tecniche

dot blot: suitable
immunocytochemistry: suitable
inhibition assay: suitable (peptide)
western blot: suitable

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

monomethylation (Lys9)

Informazioni sul gene

human ... HIST1H3F(8968)

Descrizione generale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificità

Broad species cross-reactivity is expected based on sequence homology.
This antibody recognizes Histone H3 methylated at Lys9.

Immunogeno

Epitope: Lys9
KLH-conjugated branched peptide corresponding to human Histone H3 methylated at Lys9.

Applicazioni

Anti-monomethyl-Histone H3 (Lys9) Antibody, Trial Size is a rabbit polyclonal for detection of Histone H3 monomethylated on lysine 9. Also known as H3K9me1, this antibody has dot blot proven specificity, and has been validated in WB, ICC, PIA. Small pack size is ideal for screening.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in HeLa and A431 cells.

Peptide Inhibition Analysis: 1:10,000 dilution from a representative lot was used with HeLa acid extract for peptide block analysis.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Qualità

Evaluated by Western Blot in HeLa acid extract lysate.

Western Blot Analysis: 0.1 µg/ml of this antibody detected Histone H3 on 10 µg of HeLa acid extract lysate.

Descrizione del bersaglio

~ 17 kDa

Linkage

Replaces: 07-395

Stato fisico

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
HeLa acid extract lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Yichen Ge et al.
Toxicology and applied pharmacology, 355, 164-173 (2018-07-04)
Arsenic is a known potent risk factor for bladder cancer. Increasing evidence suggests that epigenetic alterations, e.g., DNA methylation and histones posttranslational modifications (PTMs), contribute to arsenic carcinogenesis. Our previous studies have demonstrated that exposure of human urothelial cells (UROtsa

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