AB6017
Anti-F-actin-capping protein subunit beta Antibody
from rabbit
Sinonimo/i:
capping protein (actin filament) muscle Z-line, beta, F-actin capping protein beta subunit
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
purified antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Reattività contro le specie
mouse, rat, human
Reattività contro le specie (prevista in base all’omologia)
canine (based on 100% sequence homology), primate (based on 100% sequence homology), bovine (based on 100% sequence homology)
tecniche
immunocytochemistry: suitable
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
bovine ... Capzb(338052)
dog ... Capzb(478209)
human ... CAPZB(832)
mouse ... Capzb(12345)
rat ... Capzb(298584)
Descrizione generale
F-actin-capping-protein subunit beta (CapZ beta) belongs to the F-actin capping protein family, which are characteristic heterodimers, consisting of an α subunit (31-36 kDa) and a β subunit (28-32 kDa), that cap the barbed end of actin filaments within all eukaryotes. Their ability to bind the actin filaments is in a manner independent of Ca2++ and requires the C-terminal end of both subunits for optimal binding. F-actin capping protein subunit beta is contained within the Z-discs of striated muscle, where it functions to inhibit the polymerization and depolymerization of actin. Within vertebrates, there are three isoforms for each of F-actin capping protein subunit beta’s subunits. While there is almost nothing known regarding the β3 subunit, the β1 and β2 isoforms each have distinct roles. The isoform β2 is predominantly expressed in non-muscle cells and is found in intercalated discs and the cell periphery, but is not observed at Z-discs. Isoform β1 is more greatly expressed in striated muscle and is localized at the Z-discs. It also contains the COOH-terminal extension necessary for capping the actin.
Specificità
The antibody recognizes F-actin-capping protein subunit beta at the C-terminus of isoform 2.
Immunogeno
Epitope: C-terminus of Isoform 2
KLH-conjugated linear peptide corresponding to human F-actin-capping protein subunit beta of the C-terminus of isoform 2.
Applicazioni
Detect F-actin-capping protein subunit beta using this Anti-F-actin-capping protein subunit beta Antibody validated for use in WB & IC.
Immunocytochemistry Analysis: 1:500 dilution of the antibody has been shown to detect F-actin-capping protein subunit beta in NIH/3T3 and A431 cells.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Qualità
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected F-actin-capping protein subunit beta in 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected F-actin-capping protein subunit beta in 10 µg of HeLa cell lysate.
Descrizione del bersaglio
~ 34 kDa observed MW. There are 3 isoforms produced by alternative splicing: Isoform 1 at 31 kDa, Isoform 2 at 31 kDa, and Isoform 3 (sequence not available).
Stato fisico
Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.
Stoccaggio e stabilità
Stable for 1 year at 2-8°C from date of receipt.
Risultati analitici
Control
HeLa cell lysate
HeLa cell lysate
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Shamim A Sinnar et al.
Molecular biology of the cell, 25(14), 2152-2160 (2014-05-16)
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Ying-Hsi Lin et al.
Journal of muscle research and cell motility, 36(4-5), 329-337 (2015-10-03)
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Nature communications, 10(1), 386-386 (2019-01-25)
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