71194-M

0.5 M THP Solution

Reducing agent compatible with His•Bind chromatography

• Sinonimo/i: Tris(3-hydroxypropyl)phosphine
• Codice UNSPSC: 41106511
• NACRES: NA.77

Proprietà

• Livello qualitativo: 400
• Stato: solution
• Produttore/marchio commerciale: Novagen^®
• Condizioni di stoccaggio: OK to freeze
• tecniche: protein purification: suitable
• applicazioni: sample preparation
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Reducing agent compatible with HIS-BIND^® chromatography

THP [Tris(hydroxypropyl)phosphine; C₉H₂₁O₃P] is a water-soluble, odorless, sulfhydryl reducing agent that is more stable and effective than dithiothreitol (DTT). Against oxidized glutathione, the reducing capacity of THP (0.232 meq/g) is greater than that of TCEP (0.182 meq/g). THP is also more resistant to air oxidation than DTT and is compatible with immobilized metal affinity chromatography with HIS-BIND^® and Ni-NTA His•Bind resins at concentrations up to 1.0 mM. The common working concentration of THP for many applications is 15 mM. THP is provided as a ready-to-use 0.5 M solution in water.

Purity: >80% by NMR. CAS: 4706-17-6. MW: 208.2.

Caratteristiche e vantaggi

Compatability with common methods for purifying His-tagged proteins - immobilized metal affinity chromatography with HIS-BIND^® and Ni-NTA His•Bind resins at concentrations up to 1.0 mM

Stability: More stable and effective than traditional reducing agents like dithiothreitol (DTT)

Reducing Capacity: greater reducing capacity than TCEP against oxidized glutathione

Ready-to-use: THP is provided as a ready-to-use 0.5 M solution in water, making it easy to use in many applications with flexible usage

Attenzione

Toxicity: Corrosive (L)

Altre note

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Note legali

HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS05
• Avvertenze: Danger
• Indicazioni di pericolo: H314
• Consigli di prudenza: P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 - P405
• Classi di pericolo: Eye Dam. 1 - Skin Corr. 1B
• Codice della classe di stoccaggio: 8B - Non-combustible corrosive hazardous materials
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable