5-Lipoxygenase, Human, Recombinant, S. fruigiperda
5-Lipoxygenase, Human, Recombinant, S. frugiperda E.C 1.13.11.34, catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4.
Sinonimo/i:
5-LO
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Recombinant, human 5-Lipoxygenase expressed in S. fruigiperda insect cells using a baculovirus overexpression system. 5-LO catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4. It is localized in the cytosol of resting human and rat peripheral blood neutrophils, whereas it is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA2.
Attenzione
Toxicity: Standard Handling (A)
Definizione di unità
One unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25°C in 50 mM Tris-HCl, 100 µM arachidonate, 2 mM CaCl₂, 1 mM ATP, 250 µM oxygen. Oxygen consumption can be measured using a oxygraph equipped with a Clark oxygen electrode.
Stato fisico
In 100 mM Tris containing 5 mM EGTA, pH 8.0.
Ricostituzione
Following initial thaw, aliquot and freeze (-70°C).
Altre note
Brock, T.G., et al. 1997. J. Biol. Chem.272, 8276. Pouliot, M., et al. 1996. Eur. J. Biochem.238, 250. Zhang, Y.Y., et al. 1993. J. Biol. Chem.268, 2535. Shimizu, T., et al. 1984. Proc. Natl. Acad. Sci. USA81, 689.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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