344932

Furin, Human, Recombinant

• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Stato: liquid
• Livello qualitativo: 100
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

Recombinant, human furin expressed in vaccinia virus (VV:hFUR713t) infected BSC-40 cells. Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like pro-hormone convertase (PC) family. Members of this family cleave pro-hormones, neuropeptide precursors, some viral proteins, and bacterial toxins. The preparation was subjected to UV irradiation for 5 minutes to inactivate any residual virus.

Recombinant, human furin expressed in vaccinia virus (VV:hFUR713t) infected BSC-40 cells. Supplied as a crude preparation of soluble furin obtained from cytoplasmic extracts. Furin is a type I membrane protein that processes stromelysin-3 (MMP-11) and MT1-MMP. It cleaves pro-hormones, neuropeptide precursors, some viral proteins, and bacterial toxins.

This preparation was subjected to UV irradiation for 5 min to inactivate any residual vaccinia virus.

Attenzione

Toxicity: Standard Handling (A)

Definizione di unità

One unit is defined as the amount of furin that will cleave 1.0 pmol AMC from the substrate pGlu-Arg-Thr-Lys-Arg-AMC per min at 30°C, pH 7.5.

Stato fisico

In 20 mM Bis-Tris buffer, 1 mg/ml BSA, pH 7.0.

Ricostituzione

Following initial thaw, aliquot and freeze (-70°C).

Altre note

Bassi, D.E., et al. 2001. Mol. Carcinog.31, 224.
Bassi, D.E., et al. 2001. Proc. Natl. Acad. Sci. USA98, 10326.
Creemers, J.W., et al. 2001. J. Biol. Chem.276, 4211.
Wang, X., and Pei, D. 2001. J. Biol. Chem.276, 35953.
Molloy, S.S., et al. 1992. J. Biol. Chem.267, 16396.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable