189490

3-MA

≥95% (HPLC), powder, autophagy inhibitor, Calbiochem^®

• Sinonimo/i: Autophagy Inhibitor, 3-MA, 3-Methyladenine
• Formula empirica (notazione di Hill): C₆H₇N₅
• Numero CAS: 5142-23-4
• Peso molecolare: 149.15
• Numero MDL: MFCD00010531
• Codice UNSPSC: 12352200
• NACRES: NA.77

Proprietà

• Nome del prodotto: Autophagy Inhibitor, 3-MA, Autophagy Inhibitor, 3-MA, CAS 5142-23-4, is a cell-permeable autophagic sequestration blocker. Acts as an inhibitor of III PI3-Kinase.
• Livello qualitativo: 100
• Saggio: ≥95% (HPLC)
• Stato: powder
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; protect from light
• Colore: white to faint yellow
• Solubilità: DMSO: 10 mg/mL; water: 5 mg/mL
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: N1(C2=NC=NC2=C(N=C1)N)C
• InChI: 1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3; FSASIHFSFGAIJM-UHFFFAOYSA-N

Descrizione

Descrizione generale

A widely used cell-permeable autophagic sequestration blocker that effectly protects cerebellar granule cells from apoptosis following serum/potassium deprivation. Although a known class III PI3K inhibitor (IC₅₀ = 4.5 mM in cell-free enzymatic assays), 3-MA exhibits quite distinct pharmacological properties from those of two other PI3K inhibitors, LY 294002 (Cat. Nos. 440202 and 440204) and Wortmannin (Cat. No. 681675)

Confezionamento

Packaged under inert gas

Attenzione

Toxicity: Standard Handling (A)

Nota sulla preparazione

In applications where high working concentrations are necessary (e.g. 10 mM), the use of a pre-made stock solution may result in the introduction of higher than desired solvent (e.g., DMSO) into the experimental systems. In such cases, it is recommended that the solid compound be weighed out at the time of use and reconstituted directly into the solutions used in the experimental system (buffers, media, etc.). Vortexing may be required for complete solubilization in water.

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.

Altre note

Canu, N., et al. 2005. J. Neurochem.92, 1228.
Hirosako, K., et al. 2004. Biochem. Biophys. Res. Commun.316, 845.
Beugnet, A., et al. 2003. Biochem. J.372, 555.
Eskelinen, E.L., et al. 2002. Traffic3, 878.
Petiot, A., et al. 2000. J. Biol. Chem.275, 992.
Seglen, P.O. and Gordon, P.B., 1982. Proc. Natl. Acad. Sci. USA79, 1889.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable