14-420
MEK1 Protein, inactive, 50 g
Unactive, N-terminal GST & C-terminal His6-tagged, recombinant full-length human MEK1, for use in Kinase Assays.
Sinonimo/i:
MEK1 Protein
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About This Item
Codice UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.41
Prezzi e disponibilità al momento non sono disponibili
Prodotti consigliati
Origine biologica
human
Livello qualitativo
Ricombinante
expressed in E. coli
PM
Mw 71 kDa
Produttore/marchio commerciale
Upstate®
tecniche
activity assay: suitable (kinase)
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
Informazioni sul gene
human ... MAP2K1(5604)
Descrizione generale
Research area: CELL SIGNALING
N-terminal GST, and C-terminal His6-tagged, recombinant full-length human MEK1
Product Source: expressed in E.coli
MEK1, also known as Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1), is a protein kinase located on chromosome 15q22.1-q22.33, consisting of 12 exons and 393 amino acids. [1] The protein is made up of two lobes containing several conserved α-helices and β-strands. [2]
N-terminal GST, and C-terminal His6-tagged, recombinant full-length human MEK1
Product Source: expressed in E.coli
MEK1, also known as Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1), is a protein kinase located on chromosome 15q22.1-q22.33, consisting of 12 exons and 393 amino acids. [1] The protein is made up of two lobes containing several conserved α-helices and β-strands. [2]
Applicazioni
Mitogen-Activated Protein Kinase Kinase 1 has been used in in vitro kinase assays:
- to elucidate the underlying mechanisms involved in the response to Rapidly Accelerated Fibrosarcoma (RAF) inhibitors and the interactions with downstream pathways. [3]
- to investigate the role of Mitogen-Activated Protein Kinase Kinase Kinase 2 (MEKK2) as a MAP3K in the Extracellular Signal-Regulated Kinase (ERK) pathway in osteoblasts and to assess its potential as a therapeutic target for treating Neurofibromatosis Type 1 (NF1).[4]
- to assess the effectiveness of the combined targeted therapy with Dabrafenib and Trametinib in treating non-small cell lung cancers (NSCLCs). [5]
Azioni biochim/fisiol
Protein Target: MEK1
Target Sub-Family: STE Mitogen-Activated Protein Kinase Kinase 1 (MEK1) is involved in the RAS-RAF-MEK-ERK signaling pathway. This pathway plays a crucial role in various cellular processes, including apoptosis, cell cycle progression, migration, differentiation, metabolism, and proliferation. [2] One of its important functions is the phosphorylation of specific sites in extracellular signal-regulated kinases (ERKs) Mitogen-Activated Protein Kinase /Extracellular Signal-Regulated Kinase (MAPK3/ERK1 and MAPK1/ERK2). [1] MEK activation is closely associated with stimulating cell growth and transformation processes. [6]
Target Sub-Family: STE Mitogen-Activated Protein Kinase Kinase 1 (MEK1) is involved in the RAS-RAF-MEK-ERK signaling pathway. This pathway plays a crucial role in various cellular processes, including apoptosis, cell cycle progression, migration, differentiation, metabolism, and proliferation. [2] One of its important functions is the phosphorylation of specific sites in extracellular signal-regulated kinases (ERKs) Mitogen-Activated Protein Kinase /Extracellular Signal-Regulated Kinase (MAPK3/ERK1 and MAPK1/ERK2). [1] MEK activation is closely associated with stimulating cell growth and transformation processes. [6]
Qualità
routinely evaluated by phosphorylation of MBP (Catalog # 13-104) substrate in vitro
Stato fisico
glutathione-agarose followed by Ni2+/NTA-agarose
Stoccaggio e stabilità
6 months at -70°C
Altre note
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Note legali
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Skin Sens. 1
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
MEK1/2 dual-specificity protein kinases: Structure and regulation
Roskoski R Jr.
Biochemical and Biophysical Research Communications, 417(1), 5-10 (2012)
Jeeyun Lee et al.
Journal of the National Cancer Institute, 103(8), 674-688 (2011-03-15)
Metastatic colorectal cancer (CRC) patients with v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations are resistant to treatment with cetuximab, a monoclonal antibody that targets the epidermal growth factor receptor. Statins have reported antitumor activity, but it is unknown
MEKK2 mediates aberrant ERK activation in neurofibromatosis type I
Bok S, et al.
Nature Communications, 11(1), 5704-5704 (2020)
Non-V600 BRAF mutations recurrently found in lung cancer predict sensitivity to the combination of Trametinib and Dabrafenib
Noeparast A, et al.
Oncotarget, 8(36), 60094-60108 (2016)
Langerhans Cell Histiocytosis
Diagnostic Pathology (2016)
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