05-1064

Anti-Phospho-Stat1 (Tyr701) Antibody, clone 5C9.2

clone 5C9.2, from mouse

• Sinonimo/i: Transcription factor ISGF-3 components p91/p84, signal transducer and activator of transcription 1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 5C9.2, monoclonal
• Reattività contro le specie: rat, mouse, human
• tecniche: western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_009330
• N° accesso UniProt: P42224
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: phosphorylation (pTyr701)
• Informazioni sul gene: human ... STAT1(6772) ; mouse ... Stat1(20846)

Descrizione

Descrizione generale

STATs (Signal-Transducing Activators of Transcription) are the principle substrates of JAK kinases, and mediators of cytokine signaling. STAT proteins have SH2 domains, and once phosphorylated by JAK kinases, STATs dimerize in a head-to-tail fashion, through their SH2 domains. STAT1 is a critical mediator of IFN signaling, STAT4 is specific to IL-12, STAT6 is activated primarily by IL-4 and IL-13, while STAT5 A and B appear to be most important to the function of growth hormone and prolactin. STAT3 is involved in multiple signaling pathways, and mice deficient in it are not viable. In addition to tyrosine phosphorylation, many STAT proteins are targets of Ser/Thr kinases, and those phosphorylation events potentiate STAT-induced transcription. The phosphorylation state of STATs can be monitored by phosphorylation state-specific antibodies.

Specificità

This antibody recognizes phosphorylated STAT1 at tyrosine 701.

Immunogeno

Epitope: Phosphorylated Tyr701

KLH-conjugated linear peptide corresponding to the region around phosphorylated tyrosine 701.

Applicazioni

Anti-Phospho-Stat1 (Tyr701) Antibody, clone 5C9.2 detects level of Phospho-Stat1 (Tyr701) & has been published & validated for use in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Qualità

Routinely evaluated by western blot on EGF treated and untreated A431 cell lysate.

Western Blot Analysis: 0.5-2 µg/mL of this lot was used to detect STAT1 phosphorylated at Tyr701 in EGF treated and untreated A431 cell lysates.

Descrizione del bersaglio

88-92 kDa

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS and 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8ºC from date of receipt.

Risultati analitici

Control
EGF treated and untreated A431 cell lysates, PDGF treated and untreated NIH/3T3 cell lysates, pervanadate treated and untreated PC12 cell lysates.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable