100600C

E. coli Extract Polar

Avanti Research^™ - A Croda Brand

• Sinonimo/i: E. coli Polar Lipid Extract
• Numero CAS: 1240502-50-4
• Numero MDL: MFCD22495329
• Codice UNSPSC: 12352211
• NACRES: NA.25

Proprietà

• Stato: chloroform solution
• Confezionamento: pkg of 2 × 20 mL (100600C-1g); pkg of 1 × 4 mL (100600C-100mg); pkg of 5 × 4 mL (100600C-500mg)
• Produttore/marchio commerciale: Avanti Research^™ - A Croda Brand
• Concentrazione: 25 mg/mL (100600C-100mg); 25 mg/mL (100600C-1g); 25 mg/mL (100600C-500mg)
• Tipo di lipide: lipid extracts
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated. Polar lipid extract is the total lipid extract precipitated with acetone and then extracted with diethyl ether.

Applicazioni

E. coli Extract Polar is suitable to prepare small unilamellar vesicles, to mimic the surface of the E. coli inner membrane.[1]

E. coli extract polar has been for the preparation of liposomes.[2][3][4]

E. coli Extract Polar has been used:

• in unilamellar liposomes preparation for kinetic studies[5]
• in the reconstitution of ATP-binding cassette (ABC) transporter for electron paramagnetic resonance spectroscopy studies[4]
• in the reconstitution of molybdate transport protein (MolBC) for continuous wave-electron paramagnetic resonance studies[6]

Azioni biochim/fisiol

E.coli Polar lipid extracts comprise phosphatidylethanolamine as major lipid component. The minor components include the phosphatidylglycerol (PG) and cardiolipin. The leaflets of cytoplasmic membrane and inner leaflet of outer membrane are the source of E. coli lipid extract. [7] It is used for reconstituting membrane proteins[7] and forms bilayer by vesicle fusion.[8]

Confezionamento

30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (100600C-1g)

5 mL Clear Glass Sealed Ampule (100600C-100mg)

5 mL Clear Glass Sealed Ampule (100600C-500mg)

Note legali

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Informazioni sulla sicurezza

• Pittogrammi: GHS06,GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H302,H315,H319,H331,H336,H351,H361d,H372,H412
• Consigli di prudenza: P201 - P273 - P301 + P312 + P330 - P302 + P352 - P304 + P340 + P311 - P308 + P313
• Classi di pericolo: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
• Organi bersaglio: Central nervous system, Liver,Kidney
• Codice della classe di stoccaggio: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash