The UPS FASTA file is accessible from the link below:
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/protein-biology/protein-mass-spectrometry/ups1-and-ups2-proteomic
UPS2
Proteomics Dynamic Range Standard Set
Protein Mass Spectrometry Calibration Standard
Synonyme(s) :
Dynamic Range Standards, Proteomics Standard Set
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1 SET
1 160.00 CHF
5 X 1 SET
3 590.00 CHF
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1 SET
1 160.00 CHF
5 X 1 SET
3 590.00 CHF
About This Item
Produits recommandés
Source biologique
human
Niveau de qualité
Forme
ready-to-use solution
Qualité
Protein Mass Spectrometry Calibration Standard
Concentration
10.6 μg/ampule protein
Technique(s)
mass spectrometry (MS): suitable
Conditions d'expédition
wet ice
Température de stockage
−20°C
Catégories apparentées
Description générale
The Proteomics Dynamic Range Standard Set is produced from a mixture of 48 individual human source or human sequence recombinant proteins, each of which has been selected to limit heterogeneous post-translational modifications (PTMs). The protein standard is formulated from 6 mixtures of 8 proteins to present a dynamic range of 5 orders of magnitude, ranging from 50 pmoles to 500 amoles. Each protein has been quantitated by amino acid analysis (AAA) prior to formulation.
Application
Proteomics Dynamic Range Standard Set has been used in the intensity-based absolute quantification (iBAQ) of E .coli proteins,[1] embryonic stem cells (ESCs) and neuronal precursor cells (NPCs) proteomes.[2] It has also been used as a standard to spike HeLa cells for label-free quantification.[3]
The Proteomics Dynamic Range Standard Set can be used to standardize and/or evaluate mass spectrometric (e.g., LC-MS/MS, MALDI-TOF-MS, etc.) and electrophoretic analysis conditions prior to the analysis of complex protein samples. UPS2 can be used to bracket precious experimental data sets between runs of a known complex standard sample. This allows confirmation of the robustness of the analysis method and stability of the instrument employed. Additionally, laboratories generating or comparing mass spectrometric data derived from poorly defined samples can use UPS2 as an external reference to assist with the evaluation of results and experimental methodology.
Proteomics Dynamic Range Standard Set has been used for the quantification of dynamic range universal protein standard on Orbitrap Analyzer using all ion fragmentation.[4] It has been used as a standard for intensity-based absolute quantification of proteins (iBAQ) in LC-MS (liquid chromatography-mass spectrometry)/MS analysis.[5][1]
Proteomics Dynamic Range Standard Set has been used for the quantification of dynamic range universal protein standard on Orbitrap Analyzer using all ion fragmentation.[4] It has been used as a standard for intensity-based absolute quantification of proteins (iBAQ) in LC-MS (liquid chromatography-mass spectrometry)/MS analysis.[5][1]
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
- T6567Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μgFDS
Produit(s) apparenté(s)
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
Erik Ahrné et al.
Proteomics, 13(17), 2567-2578 (2013-06-25)
There is a great interest in reliable ways to obtain absolute protein abundances at a proteome-wide scale. To this end, label-free LC-MS/MS quantification methods have been proposed where all identified proteins are assigned an estimated abundance. Several variants of this
An interaction landscape of ubiquitin signaling
Zhang X, et al.
Molecular Cell, 65(5), 941-955 (2017)
Boumediene Soufi et al.
Frontiers in microbiology, 6, 103-103 (2015-03-06)
We set out to provide a resource to the microbiology community especially with respect to systems biology based endeavors. To this end, we generated a comprehensive dataset monitoring the changes in protein expression, copy number, and post translational modifications in
Christina Ludwig et al.
Molecular & cellular proteomics : MCP, 11(3), M111-M111 (2011-11-22)
For many research questions in modern molecular and systems biology, information about absolute protein quantities is imperative. This information includes, for example, kinetic modeling of processes, protein turnover determinations, stoichiometric investigations of protein complexes, or quantitative comparisons of different proteins
Andrew F Jarnuczak et al.
Journal of proteome research, 15(9), 2945-2959 (2016-07-28)
Quantitative mass spectrometry-based proteomics of complex biological samples remains challenging in part due to the variability and charge competition arising during electrospray ionization (ESI) of peptides and the subsequent transfer and detection of ions. These issues preclude direct quantification from
Articles
High-throughput proteomics advances with improved analysis methods and mass spectrometry.
Contenu apparenté
Standardize research with Universal and Dynamic Proteomics Standards, complex and well-characterized reference standards for mass spectrometry.
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Regarding SKU# UPS2, can you please send me FASTA file of 48 proteins in UPS2 product? thanks you
1 answer-
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