The product is a native protien from Arthrobacter globiformis. This material has not been sequenced. However, this information is published at UniProt.
Please see the link below to review:
https://www.uniprot.org/uniprot/D0VWQ1
U7128
Uricase from Arthrobacter globiformis
lyophilized powder, 15-30 units/mg protein (biuret)
Synonyme(s) :
Urate oxidase, Urate:O2 oxidoreductase
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656.00 CHF
About This Item
Produits recommandés
Forme
lyophilized powder
Activité spécifique
15-30 units/mg protein (biuret)
Poids mol.
tetramer ~32 kDa
Température de stockage
−20°C
Chaîne SMILES
[P](=O)([O-])(OC3C(OC(C3O)N4C=CC(=O)NC4=O)CO)OCC1OC(C(C1O)O)N2C=CC(=O)NC2=O.[N+H4]
InChI
1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
Clé InChI
VPXDHZMLJOJKOX-UHFFFAOYSA-N
Description générale
Research area: Cell Signalling. Uricase is made of four identical subunits and is a homotetrameric enzyme. It is found as a functional uricase in several organisms including, bacteria, archaea, and eukaryotes. Nonetheless, primates and humans lack a functional uricase enzyme.[1]
Application
Actions biochimiques/physiologiques
Arthrobacter globiformis is a major producer of uricase or urate oxidase in poultry manure and litter. Uricase hydrolyzes uric acid and causes ammonia volatilization in poultry manure.[3] In the majority of mammals, uricase converts uric acid to allantoin, a soluble excretion product. Humans lack uricase due to its loss during the evolution of hominids.[4] This increases the risk of humans towards gout, kidney stones, hypertension, and diabetes due to increased circulating levels of uric acid.[5]Uricase is an important enzyme that plays a role in the purine metabolic pathway.[6]
Molecular weight: 4 × ~32 KDa (tetramer)
Isoelectric point: is 4.64
KM value (uric acid as substrate): ~ 0.01 mM
The optimum pH for activity: 8.5-9.5
The optimum pH for stability: 6.0-9.5
Isoelectric point: is 4.64
KM value (uric acid as substrate): ~ 0.01 mM
The optimum pH for activity: 8.5-9.5
The optimum pH for stability: 6.0-9.5
Zn and Cu were shown to inhibit the activity of uricase. [7]
Définition de l'unité
One unit will convert 1.0 μmole of uric acid to allantoin per min at pH 9.0 at 25 °C.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Hello, I would like to get theprotein sequence of the Arthrobacter globiformis urucase, catalog U7128. Is it a recombinant enzmye made in E. coli, or is it a native prurified protein from the A. globiformis host. Thank you. Guillaume
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