Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B can be used with both adherent and suspension culture.The advantage of layering the fixative directly onto the media is that this allows the assay to work for cells in suspension. Otherwise, you need to centrifuge, remove the media and then fix. Also, it is possible to loose cells when you remove the media. It was found that adding the fixative works for the assay and the protocol was changed in 2003.
TOX6
In Vitro Toxicology Assay Kit, Sulforhodamine B based
Synonyme(s) :
biomass viability assay, sulforhodamine B, total biomass assay
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1 KIT
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1 KIT
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.84
379.00 CHF
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Produits recommandés
Forme
liquid
Utilisation
1 kit sufficient for 1,000 tests
Conditionnement
pkg of 1 kit
Conditions de stockage
dry at room temperature
λmax
565 nm
Application(s)
cell analysis
detection
Méthode de détection
colorimetric
Température de stockage
room temp
Description générale
The Sulforhodamine B method is simple, accurate, and provides consistent results. This assay system is a means of measuring total biomass by staining cellular proteins with sulforhodamine B.
Application
In Vitro Toxicology Assay Kit, Sulforhodamine B based has been used for spectrophotometric measurement of biomass (viable and non-viable cells) by total protein.[1][2] It has also been used to study the effect of 17-allylamino-17-demethoxygeldanamycin (17-AAG) on proliferation of the five uveal melanoma cell lines.[3] Absorbance of dye is measured at a wavelength of 565nm.
Actions biochimiques/physiologiques
Dye binds to cellular protein and is then solubilized in base.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A
Code de la classe de stockage
3 - Flammable liquids
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We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the
Articles
Tests sur cellules pour l'étude de la prolifération (BrdU, MTT, WST1), de la viabilité et de la toxicité cellulaires pour la recherche sur le cancer, la recherche en neurosciences et la recherche sur cellules souches.
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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Can you use Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based with suspension cells?
1 answer-
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When using Product TOX6, In Vitro Toxicology Assay Kit Sulforhodamine B based, what happens if I add kit component Product T5413, 10 mM Tris Base Solution, prior to fixing the cells?
1 answer-
If the 10 mM Tris Base Solution, Product T5413 is added to the cells before the TCA (fixative), the cells will be lysed. This reagent is not buffered and is not isotonic. The assay cannot be rescued if this occurs.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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