Stock solutions remain stable for a period of up to two weeks when stored at 4°C.
T0665
Holo-transferrine human
powder, BioReagent, suitable for cell culture, ≥97%
Synonyme(s) :
Sidérophiline, saturée en fer
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50 MG
160.00 CHF
100 MG
272.00 CHF
500 MG
930.00 CHF
1 G
1 700.00 CHF
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Changer de vue
50 MG
160.00 CHF
100 MG
272.00 CHF
500 MG
930.00 CHF
1 G
1 700.00 CHF
About This Item
Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.75
Produits recommandés
Source biologique
human plasma
Niveau de qualité
Description
Iron-saturated
Gamme de produits
BioReagent
Pureté
≥97%
Forme
powder
Technique(s)
cell culture | mammalian: suitable
Impuretés
HIV, HBsAg and HCV, none detected (tested by FDA approved testing methods)
endotoxin, tested
Solubilité
H2O: 50 mg/mL
Numéro d'accès UniProt
Conditions d'expédition
ambient
Température de stockage
2-8°C
Informations sur le gène
human ... TF(7018)
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Application
La transferrine est la principale molécule de fixation et d′administration cellulaire du fer présente dans le sérum. Les systèmes de culture cellulaire sans sérum nécessitent un système d′administration du fer. La transferrine est la forme privilégiée pour l′administration du fer car les cellules traitent le fer lié à la transferrine d′une manière appropriée sur le plan physiologique, grâce aux récepteurs de la transferrine situés à leur surface. L′holo-transferrine humaine est une transferrine de haute affinité qui peut être utilisée avec un large éventail de types de cellules. Elle est chargée en fer avant utilisation et peut être ajoutée directement aux milieux de culture cellulaire à faible teneur en fer ou dépourvus de fer.
Actions biochimiques/physiologiques
Fournit du fer aux cellules sous une forme sans danger et stable sur le plan physiologique.
Notes préparatoires
Traitée thermiquement à 60 °C (minimum) pendant au moins 10 heures.
Remarque sur l'analyse
Pureté déterminée par électrophorèse sur gel d′agarose.
Teneur en fer déterminée par ICP et communiquée pour chaque lot.
Clause de non-responsabilité
Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du ministère français de la recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Protocoles
Discover our primary human hepatocytes and plating and maintenance media formulations. Explore our protocol for plateable primary hepatocyte cell culture.
Discover our protocol for thawing, plating, and forming hepatocyte spheroids from human spheroid-qualified plateable hepatocytes. Find the formulation for hepatocyte spheroid maintenance media.
Contenu apparenté
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What is the recommended storage method for stock solutions of item T0665, holo-Transferrin human?
1 answer-
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What's the process of saturation? Do the cells express directly holo-Tf with iron? Or apo-Tf reacts with iron salts?
1 answer-
The delivery of iron to cells by transferrins is a receptor-mediated and controlled process. Cells regulate the amount of iron they receive from the extracellular environment by varying transferrin receptor expression. Two iron-loaded transferrin molecules bind to one transferrin receptor. Once bound, they are transferred into acidic endosomes within the cell’s interior. At reduced pH, the iron is released and incorporated into intracellular proteins and the storage molecule ferritin. The apo-transferrin (iron-free) is recycled back to the exterior of the cell and released. Each transferrin receptor cycle can deliver up to four atoms of iron.
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Holo-Tf is described as "iron saturated", but there is a statement "Under normal circumstances, approximately one-third of transferrin iron-binding pockets are filled." I'm confused if the product I got (T0665) is 100% saturated with iron or not.
1 answer-
This product is a Holo-Transferrin form which is generally considered to be iron-saturated. The specification for iron content of this product is 0.10 - 0.35%. Conversely, Apo-Transferrin is considered to be iron deficient. Apo-transferrin product T5391 has an iron content specification of <0.005%. Each Transferrin has the capacity to bind 2 iron (Fe3+) molecules. In a circulating system, the iron content is transitory and rarely (if ever) 100% saturated. This product is tested for total iron content. The number of binding sites that are filled is not measured. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/272/520/t0665pis.pdfHelpful?
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What is the recommended holo-transferrin solvent and how should the dissolved protein be stored?
1 answer-
This product is tested for solubility in water at 50 mg/mL. Rates are expected to be comparable when dissolving in an aqueous buffer or medium. Solutions are stable for 5-10 days at 2-8°C. This product has not been evaluated for long-term solution stability. However, medium supplements containing transferrin are stable when stored in aliquots at -20°C for up to one year. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/212/699/t0665dat.pdfHelpful?
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What is the carbohydrate content of human transferrin?
1 answer-
We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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