SRP0237

PI3 kinase (p120g) Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

• Synonyme(s) : PIK3CG, Phosphoinositide-3-kinase, catalytic, gamma, p120-PI3K
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected insect cells
• Pureté: ≥90% (SDS-PAGE)
• Forme: aqueous solution
• Activité spécifique: ≥1.4 pmol/min-μg
• Poids mol.: 131 kDa
• Conditionnement: pkg of 20 μg
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: AF327656
• Numéro d'accès UniProt: P48736
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... PIK3CG(5294)

Description

Description générale

Human p120γ ( GenBank Accession No. AF327656), full length with N-terminal His tag, MW = 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Définition de l'unité

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.