SRE0055
Protease Inhibitor Cocktail Powder
Reagents designed and manufactured under cGMP controls suitable for use in an IVD application.
Synonyme(s) :
Protease Inhibitor Cocktail powder
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
bovine lung
yeast
Forme
lyophilized powder
Température de stockage
−20°C
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Spécificité
Inhibits serine, cysteine, and metallo-proteases.
Application
Optimized and tested for general use.
Protease inhibitor cocktail powder has been used in immunoblotting and immunoprecipitation.
Protease inhibitor cocktail powder has been used in immunoblotting and immunoprecipitation.
Actions biochimiques/physiologiques
This mixture contains individual components, including AEBSF at 2 mM, Aprotinin at 0.3 μM, Bestatin at 116 μM, E-64 at 14 μM, Leupeptin at 1 μM and EDTA at 1 mM. Each component has specific inhibitory properties.
AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.
Protease helps to break down proteins into soluble peptides and amino acids.
AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.
Protease helps to break down proteins into soluble peptides and amino acids.
Composants
1 bottle in serum bottle
Components
AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin
AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin
Quantité
One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.
Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.
Notes préparatoires
This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Skin Irrit. 2
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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