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P2714

Sigma-Aldrich

Protease Inhibitor Cocktail

lyophilized powder, for the inhibition of serine, cysteine and metallo-proteases, for general use, lyophilized powder

Synonyme(s) :

Protease Inhibitor Cocktail from bovine lung, Protease Inhibitor Mix, Protease inhibitor

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77
Forme:
lyophilized powder
Température de stockage:
−20°C
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Nom du produit

Protease Inhibitor Cocktail powder, for general use, lyophilized powder

Source biologique

bovine lung

Niveau de qualité

300

Forme

lyophilized powder

Température de stockage

−20°C

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Description générale

The protease inhibitor cocktail has been optimized and tested for general use. It consists of water-soluble protease inhibitors that effectively inhibit serine, cysteine, and metalloproteases. This product is supplied as a lyophilized powder.

Spécificité

Inhibits serine, cysteine, and metallo-proteases.

Application

Protease Inhibitor Cocktail powder has been used as a component:
  • in radioimmunoprecipitation assay buffer to lyse the avian crystalline lens samples for western blot analysis[1]
  • in phosphate buffer solution (PBS) for grinding recombinant protein expressed leaves to purify virus-like particles (VLPs)[2]
  • in PBS to dissolve lyophilized skin secretion sample of A. loloensis for peptide purification[3]
  • in 0.1 M NaCl solution to wash and collect skin secretions of frog[3]

Actions biochimiques/physiologiques

The components of this mixture, such as AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.

Composants

This mixture contains individual components, including:

AEBSF at 2 mM
Aprotinin at 0.3 μM
Bestatin at 116 μ
E-64 at 14 μM
EDTA at 1 mM
Leupeptin at 1 μM

Each component has specific inhibitory properties.

Attention

The lyophilized powder is stable for 4 years when stored at -20°C.

Quantité

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Notes préparatoires

This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.

Autres remarques

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

P8465

Protease Inhibitor Cocktail powder, for use with bacterial cell extracts, lyophilized powder

P8340

Cocktail d'inhibiteurs de protéases, for use with mammalian cell and tissue extracts, DMSO solution

P8215

Cocktail d'inhibiteurs de protéases, for use with fungal and yeast extracts, DMSO solution

P8849

Cocktail d'inhibiteurs de protéases, for use in purification of Histidine-tagged proteins, DMSO solution

P9599

Cocktail d'inhibiteurs de protéases, for plant cell and tissue extracts, DMSO solution

P1860

Cocktail d'inhibiteurs de protéases, for use in tissue culture media, DMSO solution

S8820

Comprimés d'inhibiteurs de protéases SIGMAFAST™, For General Use

S8830

Cocktail d′inhibiteurs de protéases SIGMAFAST™, comprimés, sans EDTA, for use in purification of Histidine-tagged proteins

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H315 - H319

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000308021
0000233200
0000159074
068M4099V
069M4108V

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Safety and immunogenicity of plant-produced African horse sickness virus-like particles in horses
Dennis SJ, et al.
Veterninary Research, 49(1), 1-6 (2018)
Five novel antimicrobial peptides from skin secretions of the frog, Amolops loloensis
Wang M, et al.
Journal of biological and chemical chronicles, 155(1), 72-76 (2010)
F Maier et al.
Osteoarthritis and cartilage, 27(9), 1382-1391 (2019-05-24)
Given the structural changes associated with the progression of Osteoarthritis (OA), we hypothesized that patterns of through-thickness, large-strain shear evolve with early-stage OA. We therefore aimed to determine whether and how patterns of shear strains change during early-stage OA to
Céline Méhats et al.
PloS one, 3(10), e3445-e3445 (2008-10-23)
Prolonged neonatal exposure to hyperoxia is associated with high mortality, leukocyte influx in airspaces, and impaired alveolarization. Inhibitors of type 4 phosphodiesterases are potent anti-inflammatory drugs now proposed for lung disorders. The current study was undertaken to determine the effects
Giorgio Ramadori et al.
Nature communications, 10(1), 3545-3545 (2019-08-09)
Tens of millions suffer from insulin deficiency (ID); a defect leading to severe metabolic imbalance and death. The only means for management of ID is insulin therapy; yet, this approach is sub-optimal and causes life-threatening hypoglycemia. Hence, ID represents a
Afficher tous les articles scientifiques apparentés

Articles

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

Contenu apparenté

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Questions

1–10 of 12 Questions  

  1. How much PIC do I need to use in salivary samples for Western Blot?

    1 answer

    1. One bottle of the lyophilized powder makes 100 mL of the cocktail. For salivary samples, a 1:500 dilution factor has been known to work well.

      Helpful?

  2. Hi Dose this product P2714, include EDTA? what proteases do it inhibit? Thanks, Camilla

    1 answer

    1. P2714 includes EDTA at 1 mM. The components of this mixture, such as AEBSF and aprotinin, act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin among others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.

      Helpful?

  3. Wie löse ich das Pulver in 100 ml aqua dest???? Wie lagere ich das dann?

    1 answer

    1. This product is packaged in a 10 mL serum vial with a crimp cap. Dissolve the contents of the vial in 10 mL of water or working buffer, then transfer to another container for dilution to 100 mL. Store the solution in working aliquots for at least 1 month -20 C, to avoid repeated freeze/thaw cycles. See the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/212/061/p2714dat-ms.pdf

      Helpful?

  4. I intend on taking 1mL samples of blood to give serum and test hormone levels in these samples. Should I add the solubilised protease inhibitor cocktail (p2714) directly to the blood or after centrifugation (which could be a few hours later)?

    1 answer

    1. A short delay may be negligible, however, it would not be detrimental to add protease inhibitors to whole blood samples if an extended delay is anticipated. This would ensure the best results. Please see the link below to review a publication of a similar nature:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6817059/

      Helpful?

  5. How much powder is in each bottle?

    1 answer

    1. The amount in each vial is 89 mg. This material is very hygroscopic. It is recommended to prepare the entire package rather than attempt to weigh out specific amounts.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. Can Product P2714, Protease Inhibitor Cocktail,be used with Nickel columns?

    1 answer

    1. No because it contains EDTA.

      Helpful?

  8. How should I dissolve and store Product P2714, Protease Inhibitor Cocktail?

    1 answer

    1. You can dissolve the product in 10 ml of water to make a 10X stock solution and then dilute 1:10 for use.  Aliquots can be stored at -20 C for 1 month.

      Helpful?

  9. How much of Product P2714, Protease Inhibitor Cocktail, needs to be used in cell lysates?

    1 answer

    1. One bottle is recommended for the inhibition of proteases present in a maximum of 20 grams of cell extract.

      Helpful?

  10. Can Product P2714, Protease Inhibitor Cocktail, be used with ELISA?

    1 answer

    1. Yes, because it is water based.

      Helpful?

1–10 of 12 Questions  

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