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SAB5300167

Sigma-Aldrich

Monoclonal Anti-GFP antibody produced in mouse

clone 4B10B2, purified immunoglobulin, buffered aqueous solution

Synonyme(s) :

N/A

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

4B10B2, monoclonal

Forme

buffered aqueous solution

Poids mol.

27 kDa

Technique(s)

direct ELISA: 1:10,000
flow cytometry: 1:200-1:400
immunohistochemistry: 1:200-1:1,000
western blot: 1:500-1:2,000

Isotype

IgG2a

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Catégories apparentées

Description générale

GFP (green fluorescence protein) is a reporter molecule which is used for checking gene expression and protein localization. GFP emits green light when it is excited with UV/blue light. The GFP fluorescence remains stable and can be detected non-invasively in living cells.

Immunogène

Purified recombinant fragment of GFP expressed in E.coli.
Mouse monoclonal antibody raised against GFP

Application

Monoclonal Anti-GFP antibody produced in mouse has been used in western blot analysis. and immunoprecipitation.

Forme physique

Purified antibody in PBS with 0.05% sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Mihir Kumar Mandal et al.
The Plant cell, 24(4), 1654-1674 (2012-04-12)
The conserved cellular metabolites nitric oxide (NO) and oleic acid (18:1) are well-known regulators of disease physiologies in diverse organism. We show that NO production in plants is regulated via 18:1. Reduction in 18:1 levels, via a genetic mutation in
TFEB Overexpression in the P301S Model of Tauopathy Mitigates Increased PHF1 Levels and Lipofuscin Puncta and Rescues Memory Deficits
Wang H, et al.
eNeuro, 3(3) (2016)
Ying Ju Li et al.
The Plant cell, 33(6), 1907-1926 (2021-03-18)
Seed size is a major factor determining crop yields that is controlled through the coordinated development of maternal and zygotic tissues. Here, we identified Arabidopsis MATERNAL EFFECT EMBRYO ARREST45 (MEE45) as a B3 transcription factor that controls cell proliferation and
Fyn/heterogeneous nuclear ribonucleoprotein E1 signaling regulates pancreatic cancer metastasis by affecting the alternative splicing of integrin ?1
Jiang P, et al.
International Journal of Oncology, 51(1), 169-183 (2017)
Type-B ARABIDOPSIS RESPONSE REGULATORs specify the shoot stem cell niche by dual regulation of WUSCHEL.
Meng W J, et al.
Plant Cell, 29(6), 1357-1372 (2017)
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