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S9396

Sigma-Aldrich

Sphingomyelinase from Bacillus cereus

buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

Synonyme(s) :

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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25 UNITS
339.00 CHF
50 UNITS
409.00 CHF

339.00 CHF

Date d'expédition estimée le02 juin 2025

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25 UNITS
339.00 CHF
50 UNITS
409.00 CHF

About This Item

Numéro CAS:
9031-54-3
Numéro de classification (Commission des enzymes):
3.1.4.12 (BRENDA, IUBMB)
Numéro MDL:
MFCD00132362
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

339.00 CHF

Date d'expédition estimée le02 juin 2025

Devis pour commande en gros

Forme

buffered aqueous glycerol solution

Niveau de qualité

200

Activité spécifique

≥100 units/mg protein (Lowry)

Poids mol.

34.2 kDa

Activité étrangère

Phospholipase C ≤1%

Température de stockage

2-8°C

Informations sur le gène

Bacillus cereus ATCC 10987 ... spH(2751530)

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Description générale

Sphingomyelinase (SMase) is a hydrolase,[1] of 34.2 kDa and its active site contain as a minimum of two magnesium atoms.[2]

Application

Sphingomyelinase from Bacillus cereus has been used:
  • in the study to analyse the effect of sphingomyelinase on Rubella virus infectivity in cell lines[3]
  • in lipoprotein modification for the generation of cholesterol crystals in vitro[4]
  • to aggregate LDL (low-density lipoprotein)[5]

Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. [6] Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems. [7]

Actions biochimiques/physiologiques

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.
Sphingomyelin phosphodiesterase (SMPD) participates in the metabolic reactions comprising sphingomyelin.[1] SMPD has the ability to prevent colorectal carcinoma and colitis.[1]

Définition de l'unité

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

Forme physique

Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000400206
0000371482
0000400206
0000371482
0000237638

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

G Pabst et al.
European biophysics journal : EBJ, 41(10), 915-929 (2012-06-05)
Biological membranes can be targets for compounds that either disrupt their barrier function or affect protein function via membrane-mediated processes. Biophysical studies on membrane-mimetic systems composed of membrane lipids have contributed substantially to our knowledge on the pertaining membrane physics
Extracellular lipids accumulate in human carotid arteries as distinct three-dimensional structures and have proinflammatory properties
Lehti S, et al.
The American Journal of Pathology, 188(2), 525-538 (2018)
Analysis of the Effect of Sphingomyelinase on Rubella Virus Infectivity in Two Cell Lines
Otsuki N, et al.
Journal of virology (2017)
Antioxidants inhibit low density lipoprotein oxidation less at lysosomal pH: A possible explanation as to why the clinical trials of antioxidants might have failed
Ahmad F and Leake D S
Chemistry and Physics of Lipids, 213, 13-24 (2018)
The Bacillus cereus group
Molecular Medical Microbiology, 1041-1078 (2015)
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Questions

1–2 of 2 Questions  

  1. what is the concentration and volumn of this product s9396-25units

    1 answer

    1. The concentration and volume of this product vary from lot to lot. The protein content and activity of this enzyme are reported on the lot specific Certificate of Analysis. The volume may be calculated using this information. For example, a given lot that reports a protein content of 1 mg/mL and an activity of 300 units per milligram protein, will have a volume of 167 uL. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/US/en/product/sigma/s9396#product-documentation

      Please see the following link to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/230/s9396dat.pdf

      Helpful?

  2. Can you tell me concentration according to volume?????? Cat No: S9396-50UN

    1 answer

    1. Prot. Content (mg Protein/ml) and Enzym. Assay (Activity U/ml) are reported in batch specific Certificate of Analysis . Please see the link below to access a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/s9396#product-documentation

      Helpful?

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