R8029

Anti-RAD17 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-S.cerevisiae homolog of RAD24
• Numéro MDL: MFCD08705510
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41
• Conjugué: unconjugated
• application: ARR; IF; IP; WB
• Clone: polyclonal
• Espèces réactives: human
• citations: 2
• Technique(s): immunoprecipitation (IP): suitable; indirect immunofluorescence: 5-10 μg/mL using HEK293-T cells fixed with methanol-acetone; microarray: suitable; western blot: 0.5-1.0 μg/mL using HeLa cell lysates

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 70 kDa (also recognizes 75 kDa)
• Espèces réactives: human
• Concentration: ~1 mg/mL
• Technique(s): immunoprecipitation (IP): suitable; indirect immunofluorescence: 5-10 μg/mL using HEK293-T cells fixed with methanol-acetone; microarray: suitable; western blot: 0.5-1.0 μg/mL using HeLa cell lysates
• Numéro d'accès UniProt: O75943
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... RAD17(5884)

Description

Description générale

RFC-like DNA damage sensor (RAD17)/checkpoint clamp loader component, a homolog of the Schizosaccharomyces pombe checkpoint gene, rad17 is located on human chromosome 5q13.2. This gene is abundantly expressed in the testis.

Spécificité

Anti RAD17 (C-terminal) specifically recognizes RAD17 (70 kDa and 75 kDa).

Immunogène

synthetic peptide corresponding to amino acids 623-640 of human Rad17 (isoform 2), conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is present in isoforms 1-4 of RAD17.

Application

Anti-RAD17 (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Actions biochimiques/physiologiques

RFC-like DNA damage sensor (RAD17)/checkpoint clamp loader component participates in DNA replication, repair and modification or chromatin remodeling. Phosphorylation of RAD17 is a critical event during checkpoint signaling in DNA-damaged cells. RAD17 has been reported in colon, breast and non-small cell lung carcinoma. It may also participate in cell cycle control.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)