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R8654

Sigma-Aldrich

Anti-RAD17 (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonyme(s) :

Anti-S.cerevisiae homolog of RAD24, Anti-hRad17

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About This Item

Numéro MDL:
MFCD08705511
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 75 kDa

Espèces réactives

mouse, rat, human

Conditionnement

antibody small pack of 25 μL

Technique(s)

immunoprecipitation (IP): suitable
western blot: 1:500-1:1,000 using HeLa cell lysates

Numéro d'accès UniProt

O75943

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... RAD17(5884)
mouse ... Rad17(19356)
rat ... Rad17(310034)

Description générale

Cell cycle checkpoint protein RAD17 is mapped to human chromosome 5q13.2 . It is also called s a replication factor C (RFC)-like DNA damage sensor protein. Eight alternative spliced transcript variants of the gene, which encode four distinct proteins, have been reported.

Immunogène

synthetic peptide corresponding to amino acids 2-15 of human RAD17 (isoform 1), conjugated to KLH via a C-terminal added cysteine residue. The immunizing peptide differs from that of isoform 2 by two amino acids.

Application

Anti-RAD17 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Actions biochimiques/physiologiques

RAD17 has been reported in breast, non-small cell lung carcinoma and breast cancer. RAD17 is a critical event during checkpoint signaling in DNA-damaged cells. Treatment of human cells with genotoxic agents induced ATM/ATR dependent phosphorylation of RAD17 at Ser635 and Ser645 , suggesting that phosphorylation of RAD17 is a critical event during checkpoint signaling in DNA-damaged cells.

Forme physique

Supplied as a solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificats d'analyse (COA)

Lot/Batch Number
076K4754

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Consulter la Bibliothèque de documents

Human Rad17 is phosphorylated upon DNA damage and also overexpressed in primary non-small cell lung cancer tissues
Wang X, et al.
Cancer research, 61(20), 7417-7421 (2001)
Basal-like Breast cancer DNA copy number losses identify genes involved in genomic instability, response to therapy, and patient survival
Weigman VJ, et al.
Breast Cancer Research and Treatment, 133(3), 865-880 (2012)
Rad17 recruits the MRE11-RAD50-NBS1 complex to regulate the cellular response to DNA double-strand breaks
Wang Q, et al.
The Embo Journal, 33(8), 862-877 (2014)
Yasunori Fukumoto et al.
The Journal of biological chemistry, 297(2), 100831-100831 (2021-06-27)
The ATR pathway is one of the major DNA damage checkpoints, and Rad17 is a DNA-binding protein that is phosphorylated upon DNA damage by ATR kinase. Rad17 recruits the 9-1-1 complex that mediates the checkpoint activation, and proteasomal degradation of
Eun Kyeong Oh et al.
International journal of oncology, 41(6), 2038-2046 (2012-10-02)
Host genomic alterations in addition to human papillomavirus (HPV) are needed for cervical precursor lesions to progress to invasive cancer because only a small

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