This product is supplied by the ATCC. The growth phase at the time of harvest is not reported by the supplier. In general, in order to maximize production and viability, cells are in log phase or beginning stationary phase at the time of harvest.
M3770
Micrococcus lysodeikticus ATCC No. 4698
suitable for substrate for the assay of lysozyme, lyophilized cells
Synonyme(s) :
Micrococcus luetus
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5 G
97.80 CHF
25 G
392.00 CHF
100 G
1 200.00 CHF
97.80 CHF
Date d'expédition estimée le30 mai 2025
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5 G
97.80 CHF
25 G
392.00 CHF
100 G
1 200.00 CHF
About This Item
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
97.80 CHF
Date d'expédition estimée le30 mai 2025
Produits recommandés
Forme
lyophilized cells
Niveau de qualité
Adéquation
suitable for substrate for the assay of lysozyme
Température de stockage
−20°C
Application
Micrococcus lysodeikticus ATCC No. 4698 has been used in a study to assess lysozyme separation by hollow-fibre ultrafiltration. [1] It has also been used in a study to investigate the encapsulation of protein drugs in biodegradable microparticles. [2]
Lysozyme lysates harvested from cultures of Micrococcus lysodeikticus were attached to sepharose and used for affinity chromatography to isolate various bacteriolytic enzymes. [3]
Actions biochimiques/physiologiques
Micrococcus luetus is a Gram-positive bacteria that is identified by the release of yellow water-insoluble pigments. This species requires succinic acid for its growth and is found to be susceptible to β-lytic metalloendopeptidase lyses by Lysobacter enzymogenes. Its membrane includes enzymes that participate in the prenylation reactions by utilizing prenyl pyrophosphates as donors. M. luteus is known to be used for cloning the cis-prenyl transferase gene.
Qualité
Contains polynucleotide phosphorylase.
Définition de l'unité
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Protocoles
To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
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From which growth phase are the bacteria harvested?
1 answer-
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