Frozen aliquots can be stored up to 2 years. For more information, please see the link below:
https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides
L4516
Lipopolysaccharides from Escherichia coli O127:B8
BioXtra, suitable for cell culture, γ-irradiated
Synonyme(s) :
LPS
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1 MG
109.00 CHF
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1 MG
109.00 CHF
About This Item
Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352211
Nomenclature NACRES :
NA.75
Produits recommandés
Source biologique
Escherichia coli (O127:B8)
Niveau de qualité
Stérilité
γ-irradiated
Gamme de produits
BioXtra
Forme
lyophilized powder
Produit purifié par
gel-filtration chromatography
Technique(s)
cell culture | mammalian: suitable
Impuretés
<1% Protein (Lowry)
Solubilité
H2O: 5 mg/mL, slightly hazy
Température de stockage
2-8°C
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Application
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Lipopolysaccharides from Escherichia coli 0127:B8 has been used:
- to study the LPS-induced macrophage survival in mice[1]
- to elicit the secretion of cytokines by cattle PBMC[2]
- to study apoptosis of highly susceptible macrophage cells in a LPS-induced proinflammatory environment[1]
- to perform autophagy assay in BV2 and BV2-LC3 cells[3]
- for stimulation of human monocytes, macrophages and mouse microglia cells[4][5][6][7]
- to induce pentraxin-3 expression in 293T cells[8]
Actions biochimiques/physiologiques
Reconstitution
Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.
Autres remarques
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 2 Oral
Code de la classe de stockage
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Certificats d'analyse (COA)
Lot/Batch Number
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Les clients ont également consulté
Li Wei et al.
Journal of neuroinflammation, 14(1), 198-198 (2017-10-05)
Several G-protein-coupled receptors (GPCRs) have been shown to be important signaling mediators between neurons and glia. In our previous screening for identification of nerve injury-associated GPCRs, G-protein-coupled receptor 84 (GPR84) mRNA showed the highest up-regulation by microglia after nerve injury.
Hua Liu et al.
International journal of clinical and experimental pathology, 6(4), 660-668 (2013-04-11)
The aim of the present study was to explore the role of receptor tyrosine kinases (RTKs) in the regulation of expression of PTX-3, a protector in atherosclerosis. Human monocytic U937 cells were infected with a shRNA lentiviral vector library targeting
Srinivas Akula et al.
Cells, 9(9) (2020-09-23)
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Hye-Eun Han et al.
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Microglia play a role in maintaining and resolving brain tissue homeostasis. In pathological conditions, microglia release pro-inflammatory cytokines and cytotoxic factors, which aggravate the progression of neurodegenerative diseases. Autophagy pathway might be involved in the production of pro-inflammatory cytokines and
A Charlotte M T de Wolf et al.
PloS one, 12(6), e0179942-e0179942 (2017-06-29)
Regulatory T cells (Treg) function in the prevention of excessive inflammation and maintenance of immunological homeostasis. However, these cells may also interfere with resolution of infections or with immune reactions following vaccination. Effects of Treg on vaccine responses are nowadays
Articles
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
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Once reconstituted what is the stability at -20C for the LPS product L4516-1M?
1 answer-
Helpful?
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Can LPS bind to plastics (ex. 1.5 mL microtube) even when LPS solution is frozen stored at -20℃? I store LPS solution at 0.05mg/mL in -20℃ now.
1 answer-
LSP is less likely to stick to plastics than glass, however very low concentrations (<0.1 mg/mL in water), LPS tends to stick to containers made
of certain types of glass or plastic. To minimize, prepare stock solutions of at least 1 mg/mL. If the LPS concentration is >1 mg/mL, adsorption to the sides of the vial is
negligible. For solutions stored in glass, vortexing for 30 minutes following thaw is recommended to redissolve. This may be useful for dilute solutions in plastic, as well. Surfaces (glass and plastics) may be salinized to reduce adherence.See Sigmacote, SL2:
https://www.sigmaaldrich.com/product/sigma/sl2For additional information, see the link below to review the Lipopolysaccharides product information page:
https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharidesHelpful?
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