KCQS00
KiCqStart® SYBR® Green qPCR ReadyMix™
For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
Synonyme(s) :
qPCR master mix, sybr green qPCR
About This Item
Produits recommandés
Forme
liquid
Utilisation
sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions
Caractéristiques
dNTPs included
hotstart
Conditions de stockage
protect from light
Technique(s)
qPCR: suitable
Couleur
colorless
Entrée
purified DNA
Compatibilité
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MiniOpticon
for use with Bio-Rad MyiQ
for use with Eppendorf® Mastercycler ep realplex2 s
for use with Eppendorf® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
Méthode de détection
SYBR® Green
Conditions d'expédition
dry ice
Température de stockage
−20°C
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Description générale
Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.
Application
- Gene expression
- DNA quantification
- CHiP
Caractéristiques et avantages
- Assay results in as little as 33 minutes
- Highly efficient and sensitive real-time PCR results
- Little/no optimization required
Composants
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
Autres remarques
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.
Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:
- Bio-Rad® CFX384™
- Bio-Rad CFX96™
- Bio-Rad MiniOpticon™
- Bio-Rad/MJ Chromo4™
- Bio-Rad/MJ Opticon 2
- Bio-Rad/MJ Opticon™
- Cepheid SmartCycler®
- Eppendorf Mastercycler® ep realplex
- Eppendorf Mastercycler ep realplex2 s
- Illumina Eco qPCR
- Qiagen/Corbett Rotor-Gene® 3000
- Qiagen/Corbett Rotor-Gene 6000
- Qiagen/Corbett Rotor-Gene Q
- Roche LightCycler® 480
Informations légales
En option
Produit(s) apparenté(s)
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Articles
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
Après une PCR classique, l'analyse des données de PCR/qPCR est effectuée par une séparation sur un gel d'agarose, ou depuis peu, à travers un capillaire.
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
Protocoles
Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.
Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.
SYBR Green I dye in qPCR measures target quantity, adaptable to specific needs with a standard protocol.
Gradient PCR optimizes assay conditions by testing fixed primer concentrations across various annealing temperatures.
Contenu apparenté
SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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