KCQS03
KiCqStart® SYBR® Green qPCR ReadyMix™
iQ™, with fluorescein for Bio-Rad systems
About This Item
Produits recommandés
Forme
liquid
Utilisation
sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions
Caractéristiques
dNTPs included
hotstart
Conditions de stockage
protect from light
Technique(s)
qPCR: suitable
Couleur
colorless
Entrée
purified DNA
Compatibilité
for use with Bio-Rad MyiQ
for use with Bio-Rad iCycler iQ
for use with Bio-Rad iQ 5
Méthode de détection
SYBR® Green
Conditions d'expédition
dry ice
Température de stockage
−20°C
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Description générale
Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.
Application
Different real-time PCR systems employ different strategies for the normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix, iQ contains fluorescein for experimental plate well factor collection on iCycler iQ real-time detection systems or the MyiQ detection system.
- Gene expression
- DNA quantification
- CHiP
Caractéristiques et avantages
- Assay results in as little as 33 minutes
- Highly efficient and sensitive real-time PCR results
- Little/no optimization required
Composants
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
Autres remarques
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.
Informations légales
En option
Produit(s) apparenté(s)
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Articles
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
Après une PCR classique, l'analyse des données de PCR/qPCR est effectuée par une séparation sur un gel d'agarose, ou depuis peu, à travers un capillaire.
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
Protocoles
Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.
Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.
SYBR Green I dye in qPCR measures target quantity, adaptable to specific needs with a standard protocol.
Gradient PCR optimizes assay conditions by testing fixed primer concentrations across various annealing temperatures.
Contenu apparenté
SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique