I1284

Isopropyl β-D-thiogalactopyranoside solution

ReadyMade IPTG solution for Blue-white screening

• Synonyme(s) : IPTG solution
• Formule empirique (notation de Hill) : C₉H₁₈O₅S
• Numéro CAS: 367-93-1
• Poids moléculaire : 238.30
• Beilstein: 4631
• Numéro MDL: MFCD00063273
• Code UNSPSC : 12352200
• ID de substance PubChem : 24895928
• Nomenclature NACRES : NA.52

Propriétés

• Qualité: Molecular Biology; for molecular biology
• Niveau de qualité: 200
• Description: non-ionic
• Forme: liquid
• Poids mol.: 238.3
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Chaîne SMILES: CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O
• InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
• Clé InChI: BPHPUYQFMNQIOC-NXRLNHOXSA-N

Description

Description générale

Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins in E. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter. IPTG Ready Made is a 0.2 μm filter sterilized solution of 200 mM IPTG. This reagent remains liquid at its storage temperature (-20 °C), thus avoiding freeze-thaw cycles and providing a user-friendly solution.

Application

Isopropyl β-D-thiogalactopyranoside solution is:

• used as an inducer for the expression of single domain antibodies(sdAbs) in E coli[1] and bioactive peptides[2]
• used in its half-life and decay studies[3]
• suitable for induction of recombinant protein expression in systems controlled by the lac repressor

Actions biochimiques/physiologiques

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. Since Isopropyl β-D-thiogalactopyranoside cannot be metabolized by E. coli its concentration remains constant. Therefore, it is not a variable in the experiment.[4]

Principe

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β-galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)