B2904

Bluo-Gal

reagent for selection of recombinant bacterial clones

• Synonyme(s) : 5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal
• Formule empirique (notation de Hill) : C₁₄H₁₆BrNO₆
• Numéro CAS: 97753-82-7
• Poids moléculaire : 374.18
• Beilstein: 1550140
• Numéro MDL: MFCD00063691
• Code UNSPSC : 12352200
• ID de substance PubChem : 24891417
• Nomenclature NACRES : NA.85

Propriétés

• Qualité: Molecular Biology; for molecular biology
• Stérilité: non-sterile
• Essai: ≥98% (HPLC)
• Forme: powder
• Technique(s): nucleic acid detection: suitable
• Solubilité: DMF: soluble
• Adéquation: suitable for substrate for β-galactosidase
• Température de stockage: −20°C
• Chaîne SMILES: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
• Clé InChI: LINMATFDVHBYOS-MBJXGIAVSA-N

Description

Description générale

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac⁺ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis,[1] Dictyostelium samples[2] and in the staining of vena cava of adult mice.[3]

Chromogenic substrate suitable for identification of lac⁺ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Principe

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)