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H6024

Sigma-Aldrich

Hoechst Stain solution

Synonyme(s) :

Bisbenzimide H 33258

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About This Item

Numéro CAS:
23491-45-4
Numéro MDL:
MFCD00012679
Code UNSPSC :
12352207
ID de substance PubChem :
24895727
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Forme

solution

Application(s)

histology

Température de stockage

2-8°C

Chaîne SMILES 

O.Cl.Cl.Cl.CN1CCN(CC1)c2ccc3NC(=NCc3c2)c4ccc5NC(=NCc5c4)c6ccc(O)cc6

InChI

1S/C27H28N6O.3ClH.H2O/c1-32-10-12-33(13-11-32)22-5-9-25-21(15-22)17-29-27(31-25)19-4-8-24-20(14-19)16-28-26(30-24)18-2-6-23(34)7-3-18;;;;/h2-9,14-15,34H,10-13,16-17H2,1H3,(H,28,30)(H,29,31);3*1H;1H2

Clé InChI

OWRSPPSBNWJJAR-UHFFFAOYSA-N

Description générale

Hoechst stains are part of a family of blue fluorescent dyes commonly used to stain DNA. It gets incorporated biosynthetically into interphase nuclei in fixed cytologic preparations and in unfixed cultured cells.[1] It can also be used to detect biosynthetic incorporation of 5-bromodeoxyuridine into the DNA of living cells. Hoechst stains property may allow fluorescence-activated cell and chromosome sorting according to the extent of DNA synthesis which may provide a bridge between biochemical and cytologic analyses of processes related to DNA replication.[2]

Application

A fluorescent DNA stain qualified for use in Mycoplama staining.

Composants

Contains 0.5μg/ml Hoechst bisbenzimide 33258 fluorochrome stain and thimerosal.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
RNBF6578
RNBF2894
RNBD7774
RNBD1408
RNBC5497

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Kin Lam Fok et al.
Endocrinology, 158(11), 4000-4016 (2017-09-25)
Spermatogenesis is sustained by a heterogeneous population of spermatogonia that includes the spermatogonial stem cells. However, the mechanisms underlying their establishment from gonocyte embryonic precursors and their maintenance thereafter remain largely unknown. In this study, we report that inactivation of
Feifan Zhou et al.
International journal of cancer, 125(6), 1380-1389 (2009-06-18)
Although anti-tumor immunological responses have been mainly associated with necrosis, apoptosis-associated immune responses have been recently suggested as well. In this study, we investigated anti-tumor immune responses and regulatory mechanisms of HSP70 using apoptotic cells induced by photodynamic therapy (PDT).
Marisa Müller et al.
PLoS biology, 9(4), e1000611-e1000611 (2011-04-29)
In eukaryotes, hundreds of mRNAs are localized by specialized transport complexes. For localization, transcripts are recognized by RNA-binding proteins and incorporated into motor-containing messenger ribonucleoprotein particles (mRNPs). To date, the molecular assembly of such mRNPs is not well understood and
Nicola Cirillo et al.
British journal of cancer, 117(7), 984-993 (2017-08-11)
Recent studies have shown that production of cortisol not only takes place in several non-adrenal peripheral tissues such as epithelial cells but, also, the local inter-conversion between cortisone and cortisol is regulated by the 11β-hydroxysteroid dehydrogenases (11β-HSDs). However, little is
Georg Tascher et al.
Cells, 8(2) (2019-02-24)
Of the hepatic cell lines developed for in vitro studies of hepatic functions as alternatives to primary human hepatocytes, many have lost major liver-like functions, but not HepaRG cells. The increasing use of the latter worldwide raises the need for
Afficher tous les articles scientifiques apparentés

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