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D8273

Sigma-Aldrich

Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA

lyophilized powder

Synonyme(s) :

single-stranded DNA-Cellulose, ss-DNA-Cellulose, DNA-Cellulose

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1 G
225.00 CHF
5 G
776.00 CHF
10 G
1 350.00 CHF

225.00 CHF

Date d'expédition estimée le27 mars 2025

Devis pour commande en gros
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Sélectionner une taille de conditionnement

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1 G
225.00 CHF
5 G
776.00 CHF
10 G
1 350.00 CHF

About This Item

Numéro MDL:
MFCD00130925
Code UNSPSC :
41106305
eCl@ss :
32160414
Nomenclature NACRES :
NA.51

225.00 CHF

Date d'expédition estimée le27 mars 2025

Devis pour commande en gros

Forme

lyophilized powder

Niveau de qualité

200

Ampleur du marquage

≥3 mg single-stranded calf thymus DNA/g

Température de stockage

−20°C

Catégories apparentées

Description générale

Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA, also known as ctDNA is a natural source of DNA designed for DNA binding agent studies. The detection limits of ctDNA are around 2.0ng and linear ranges of light scattering intensity (I(LS)) is 0.05-1.5 mµg.[1]

Application

Deoxyribonucleic acid−cellulose single-stranded from calf thymus DNA is used to study the binding parameters and binding specificity of a variety of proteins, nucleotides, and chemical reagents including anti-cancer agents. It is frequently used in pull-down assays and chromatographic procedures.

Qualité

May contain a trace of Tris and EDTA.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
SLCD5790
SLBG5813V
044H7125
021K7020
112K7010

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

D Pisetsky et al.
The Journal of rheumatology, 26(9), 1934-1938 (1999-09-24)
To elucidate the epitope structure to DNA by identifying antigenic determinants on bacterial DNA bound by anti-DNA antibodies from normal human subjects (NHS) and patients with systemic lupus erythematosus (SLE). Sera from NHS and patients with SLE were tested by
T Kuzuhara et al.
Biochemical and biophysical research communications, 362(4), 805-810 (2007-09-04)
Tumor necrosis factor-alpha (TNF-alpha) inducing protein (Tipalpha) is a carcinogenic factor secreted from Helicobacter pylori (H. pylori), mediated through both enhanced expression of TNF-alpha and chemokine genes and activation of nuclear factor-kappaB. Since Tipalpha enters gastric cancer cells, the Tipalpha
A Georgaki et al.
Nucleic acids research, 22(7), 1128-1134 (1994-04-11)
A DNA helicase from calf thymus, called DNA helicase F, copurified with replication protein A through several steps of purification including DEAE-Sephacel, hydroxyapatite and single stranded DNA cellulose. It is finally separated from replication protein A on FPLC Mono Q
Q Hua et al.
Cellular and molecular life sciences : CMLS, 60(2), 413-421 (2003-04-08)
Tau, a major microtubule-associated protein of the neuron, which is known to promote the assembly of and to stabilize microtubules, has also been seen associated with chromatin in neuronal cell lines, but its role in this subcellular compartment is still
X Du et al.
Talanta, 55(1), 93-98 (2008-10-31)
The interaction of histone with nucleic acids was characterized by light-scattering measurement using a common spectrofluorometer. Thereby, a sensitive and convenient method for the determination of nucleic acids was established. At pH 4.5-6.5, the interaction of histone with nucleic acids
Afficher tous les articles scientifiques apparentés

Questions

  1. Instructions on how to pack a ssDNA cellulose column, like how to soak the powder (buffer, for how long, and temperature). Thanks

    1 answer

    1. The powder may be reconstituted in 10 mM Tris HCl buffer (pH 7.4) containing 1 mM EDTA. One gram of powder will swell to roughly 3 milliliters of resin.
      Relatively high volumes are required to maintain a suspension; a range of 15 – 30 mL per gram of dry powder is typical.
      For additional buffer recommendations, please refer to Methods in Enzymology, volume XXI, page 199 (1971).
      Gravity flow through a disposable column is recommended for best results.
      The use of a pump may cause the cellulose to compress, resulting in high back pressure and a very slow flow rate.

      Please see the research papers linked below that describe the use of this product in purification applications.

      https://journals.asm.org/doi/pdf/10.1128/mcb.16.10.5604
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316966/

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