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D8037

Sigma-Aldrich

Driselase™ Basidiomycetes sp.

BioReagent, suitable for plant cell culture

Synonyme(s) :

Driselase™ from Basidiomycetes sp.

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About This Item

Numéro CAS:
85186-71-6
Numéro CE :
286-055-3
Numéro MDL:
MFCD00130978
Code UNSPSC :
10171502
Nomenclature NACRES :
NA.72

Gamme de produits

BioReagent

Forme

powder

Composition

Protein, ≥10% biuret

Technique(s)

cell culture | plant: suitable

Application(s)

agriculture

Température de stockage

−20°C

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Catégories apparentées

Application

Driselase™ Basidiomycetes sp. has been used:

  • in spheroplast preparation from Coccomyxa cells
  • in a CRISPR/Cas9-based mutagenesis protocol for Brachypodium distachyon and its allopolyploid relative, Brachypodium hybridum
  • for cell wall digestion to perform whole-mount immunolocalization of Lotus japonicus root tissue

Actions biochimiques/physiologiques

Driselase™ is a natural mixture of enzyme activities (fungal carbohydrates) used to digest plant cell walls to facilitate the maceration of plant materials, protoplast formation, and extraction processes. Driselase releases cell wall carbohydrates. This formulation contains enzyme activities of cellulose, endo-1,3-β-glucanase, and xylanase.

Autres remarques

Crude powder containing laminarinase, xylanase and cellulase.

Informations légales

Driselase is a trademark of ASKA Animal Health Co. Ltd.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Dieter Hackenberg et al.
Plant physiology, 172(2), 1154-1166 (2016-08-24)
In this study, we report the functional characterization of heterotrimeric G-proteins from a nonvascular plant, the moss Physcomitrella patens. In plants, G-proteins have been characterized from only a few angiosperms to date, where their involvement has been shown during regulation
M C Ralet et al.
Carbohydrate research, 263(2), 227-241 (1994-10-17)
Cell walls from sugar-beet pulp contain some feruloyl groups linked to the pectic neutral side-chains. Enzymic as well as chemical hydrolysis of the pulp yielded a series of feruloylated oligosaccharides, which have been purified by Sephadex LH-20 and Biogel P-2
D P Blowers et al.
Plant physiology, 86(2), 505-509 (1988-02-01)
Plasma membrane vesicles from wild carrot cells grown in suspension culture were isolated by aqueous two-phase partitioning, and ATP-dependent phosphorylation was measured with [gamma-(32)P]ATP in the presence and absence of calcium. Treatment of the carrot cells with the cell wall
D T Kaplan et al.
Journal of nematology, 22(3), 399-406 (1990-07-01)
Radopholus spp. were reared in carrot tissue culture via established procedures, with slight modification. Several plant tissue maceration enzymes and flotation media (salts and sucrose) were evaluated with regard to nematode toxicity and extraction efficiency. Best extraction of viable nematodes
Laura A Moody et al.
The New phytologist, 218(3), 1270-1277 (2018-03-03)
Forward genetics is now straightforward in the moss Physcomitrella patens, and large mutant populations can be screened relatively easily. However, perturbation of development before the formation of gametes currently leaves no route to gene discovery. Somatic hybridization has previously been
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