The recommended 1X reaction buffer is 20 mM Tris, pH 7.4, 100 mM NaCl, 5 mM MgCl2, 1 mM DTT. The dilution buffer, which is included in this kit, is recommended for further dilution of reconstituted proteins, if required.
CAS9PL
Cas9 Plus Protein
from Streptococcus pyogenes, recombinant, expressed in E. coli, 3X NLS
Synonyme(s) :
Cas9, SpCas9, SpyCas9
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50 μG
155.00 CHF
250 μG
560.00 CHF
Sélectionner une taille de conditionnement
Changer de vue
50 μG
155.00 CHF
250 μG
560.00 CHF
About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.51
Produits recommandés
Produit recombinant
expressed in E. coli
Niveau de qualité
Essai
≥95% (SDS-PAGE)
Forme
lyophilized powder
Conditionnement
pkg of 1 kit (4 components)
Application(s)
CRISPR
Conditions d'expédition
wet ice
Température de stockage
−20°C
Description générale
Recombinant Cas9 Plus protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, Streptococcus pyogenes Cas9 Plus protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.
Application
Functional Genomics; Target Validation; Genome Editing
Caractéristiques et avantages
- Enhanced specificity compared to wild type Cas9
- Increased activity compared to enhanced specificity Cas9
- Ready to inject/transfect
Conditionnement
pkg of 50 μg (≥ 300 pmol)
pkg of 250 μg (≥ 1500 pmol)
pkg of 250 μg (≥ 1500 pmol)
Composants
Each kit consists of:
- one vial of Cas9 Plus Recombinant Protein
- one vial of Plus Transfection Buffer
- one vial of Reconstitution Buffer
- one vial of Dilution Buffer
Principe
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
Reconstitution
Lyophilized S. pyogenes Cas9 protein should be resuspended in the Reconstitution solution provided to desired concentration. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.
Autres remarques
Use our CRISPR Selection Tool to order gRNA
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
Informations légales
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
10 - Combustible liquids
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Protocoles
Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
Contenu apparenté
Explore technology and reagent portfolios for plant breeding workflows, accelerating the development of new crop varieties.
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Hi, which reaction buffer can I use for Cas9 Plus-sgRNA complex for in vitro assay? I need to check cleavage of DNA in vitro before injections into animals and I didnt find any protocols or reccomendations in datasheets. Can I use dilution buffer?
1 answer-
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