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A8332

Sigma-Aldrich

p-Aminobenzamidine–Agarose

(1:1 suspension in a 1.0 M NaCl + 20% ethanol solution)

Synonyme(s) :

pABA

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About This Item

Numéro MDL:
MFCD00165084
Code UNSPSC :
41106514
Nomenclature NACRES :
NA.56

Source biologique

plant

Forme

(1:1 suspension in a 1.0 M NaCl + 20% ethanol solution)

Technique(s)

protein purification: suitable

Matrice

cross-linked 6% beaded agarose

Activation de la matrice

epichlorohydrin

Fixation de matrice

amino to carboxyl of spacer

Espaceur de matrice

9 atoms

Capacité

≥5 mg/mL binding capacity (trypsin)

Température de stockage

2-8°C

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Application

p-Aminobenzamidine Agarose (pABA) can be used as an affinity ligand to bind and remove serine proteases in addition to Ca2+-binding proteins. It has also been used in medical applications as a tool for the purification of non-protease proteins.

Forme physique

1:1 suspension in a 1.0 M NaCl + 20% ethanol solution

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Gulay Bayramoglu et al.
Bioprocess and biosystems engineering, 35(3), 423-431 (2011-08-20)
In this work, a new methodology is developed for selection of affinity ligands towards the enzyme "trypsin" using quartz crystals microbalance (QCM) technique. To achieve this goal, the surface amination of gold plated QCM crystals was achieved in 13.56 MHz
Ya-Chi Su et al.
The Journal of biological chemistry, 286(36), 31904-31914 (2011-07-23)
To select residues in coagulation factor XIa (FXIa) potentially important for substrate and inhibitor interactions, we examined the crystal structure of the complex between the catalytic domain of FXIa and the Kunitz protease inhibitor (KPI) domain of a physiologically relevant
Tomas Erban
PloS one, 6(8), e22860-e22860 (2011-08-31)
p-aminobenzamidine (p-ABA) is used as a ligand in the purification of many serine proteases and in their removal from heterogeneous samples. Moreover, p-ABA has a potent ability to bind Ca(2+)-binding proteins. The binding ability and use of p-ABA in purification
Paola Fortugno et al.
Journal of cell science, 115(Pt 3), 575-585 (2002-02-28)
Survivin is a member of the inhibitor of apoptosis gene family that has been implicated in both apoptosis inhibition and regulation of mitosis. However, the subcellular distribution of survivin has been controversial and variously described as a microtubule-associated protein or
Massimo D'Agostino et al.
Nature, 551(7682), 634-638 (2017-11-02)
Membrane fusion in eukaryotic cells mediates the biogenesis of organelles, vesicular traffic between them, and exo- and endocytosis of important signalling molecules, such as hormones and neurotransmitters. Distinct tasks in intracellular membrane fusion have been assigned to conserved protein systems.
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