RECOMT
Thrombin CleanCleave™ Kit
Synonyme(s) :
Thrombin Cleavage Kit
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.56
Produits recommandés
Forme
suspension
Poids mol.
37 kDa
Technique(s)
protein purification: suitable
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Description générale
The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins
Application
For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.
Caractéristiques et avantages
- Fast, efficient cleavage in as little as 2 hours
- Thrombin is covalently bound to agarose for easy removal.
- The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
- Cleave tags even in the presence of 0.1% Triton™, 1 M urea or 5 mM EDTA
- Thrombin-agarose is reusable.
Quantité
200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.
Informations légales
CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Composants de kit seuls
Réf. du produit
Description
- Thrombin Cleavage Buffer, 10× 10 mL
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Protocoles
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