This product is filled by density, with a target fill volume of 0.5 ml for the 25mg and 2.0 ml for the 100mg package sizes.
A1602
Amyloglucosidase from Aspergillus niger
ammonium sulfate suspension, ≥40 units/mg protein
Synonyme(s) :
1,4-α-D-Glucane glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase
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Sélectionner une taille de conditionnement
25 MG
125.00 CHF
100 MG
401.00 CHF
125.00 CHF
Date d'expédition estimée le16 avril 2025
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Changer de vue
25 MG
125.00 CHF
100 MG
401.00 CHF
About This Item
Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
125.00 CHF
Date d'expédition estimée le16 avril 2025
Produits recommandés
Forme
ammonium sulfate suspension
Niveau de qualité
Activité spécifique
≥40 units/mg protein
Conditions d'expédition
wet ice
Température de stockage
2-8°C
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Description générale
Application
Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle [3].
Actions biochimiques/physiologiques
Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides [6].
Définition de l'unité
One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.
Forme physique
Suspension in 3.2 M (NH4)2SO4 solution, pH approx. 6.0
Autres remarques
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
Protocoles
This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.
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What volume of ammonium sulfate is the 25mg suspended in?
1 answer-
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How can a 1mg/mL solution of amyloglucosidase A1602 be prepared in acetate buffer?
1 answer-
The product A1602 is in an ammonium sulfate solution. Here's how you can work with this product:
- If the ammonium sulfate might interfere with the reaction(s):
1. Remove the enzyme from the refrigerator, ensuring that the ammonium sulfate suspensions are not frozen.
2. Gently invert the vial or bottle to produce a milky suspension. Avoid using harsh treatments like vortexing or sonicating, as they may denature some of the enzyme.
3. Use a pipette and sterile wide-bore pipette tip to remove a portion of the suspension.
4. Transfer the portion to a clean microcentrifuge or centrifuge tube.
5. Pellet the enzyme by centrifuging at approximately 10,000 – 15,000 x g for 10 minutes, if possible, using a refrigerated microcentrifuge set to a temperature between 2 °C and 8 °C.
6. Carefully remove as much clear supernatant as possible, retaining it; it is not necessary to remove absolutely all of the ammonium sulfate solution.
7. Dissolve the pellet by adding an appropriate amount of reaction buffer.
8. Assay the supernatant and the solution from the pellet for protein content and/or enzyme activity.- If the ammonium sulfate will not interfere with the reaction(s), follow the same steps as above.
It's important to note that for an ammonium sulfate suspension, most of the enzyme will be in solid form, and only negligible amounts of enzyme will likely be in the ammonium sulfate solution.
Alternatively, there are several Amyloglucosidase enzymes available in solid form (e.g., Product A7420 and A9228) that can be suspended in a solvent of your choice.
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