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94740

Sigma-Aldrich

Vanadyl ribonucleoside complexes

BioReagent, for molecular biology

Synonyme(s) :

Ribonucleoside vanadyl complexes, VRC

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250 MG
131.00 CHF
1 G
433.00 CHF

131.00 CHF

Date d'expédition estimée le16 avril 2025

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250 MG
131.00 CHF
1 G
433.00 CHF

About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.52

131.00 CHF

Date d'expédition estimée le16 avril 2025

Qualité

for molecular biology

Niveau de qualité

100

Gamme de produits

BioReagent

Forme

powder

Adéquation

in accordance for ribonuclease inhibition test

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.[1]

Application

Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.
Vanadyl ribonucleoside complexes has been used in hybridization mixture for RNA in situ hybridization.[2][3][4]

Actions biochimiques/physiologiques

The vanadyl ribonucleoside complex has the ability to block the formation of ribosomal subunit in Staphylococcus aureus. The complex exhibits anti-microbial activity[1] These complexes also serve as powerful inhibitors of several enzymes with ribonucleolytic activity.[5]

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
BCCD5801
BCBC5675V
BCBC5675
1140341
47698/1

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Inhibitors of RNases
Sambrook, J. and Russell, D.W. et al.
Molecular Cloning: A Laboratory Manual, 1, 7-7 (2001)
Tissue array for Tp53, C-myc, CCND1 gene over-expression in different tumors
Liu G Y, et al.
World Journal of Gastroenterology, 14(47), 7199-7199 (2008)
Analysis of AC3-33 gene expression in multiple organ cancer and adjacent normal tissue by RNA in situ hybridization
Hu F, et al.
Oncology Letters, 9(6), 2795-2798 (2015)
Effect of ribonucleoside-vanadyl complexes on enzyme-catalyzed reactions central to recombinant DNA technology
Puskas R S, et al.
Biochemistry, 21(19), 4602-4608 (1982)
Isolation of cytoplasmic RNA: ribonucleoside-vanadyl complexes.
S L Berger
Methods in enzymology, 152, 227-234 (1987-01-01)
Afficher tous les articles scientifiques apparentés

Questions

1–2 of 2 Questions  

  1. Can you provide me with a protocol for preparing a solution of Vanadyl-ribonucleoside complex (94740-1G) at a concentration of 400 mM?

    Can you provide me with a protocol for preparing a solution of Vanadyl-ribonucleoside complex (94740-1G) at a concentration of 400 mM?

    1 answer

    1. This product is a complex that has an estimated molecular weight of 422.5 g/mole. The solubility at 400 mM has not been tested. This product is offered in a prepared solution under product 94742 at a concentration of 200 mM. Based on the estimated molecular weight, a 0.4 M (400 mM) solution would have a concentration of 169 mg/mL. Solutions may be prepared in nuclease-free PBS containing 15% glycerol or 5% DMSO as a cryoprotectant. Solutions may then be stored in working aliquots at −20˚C. Avoid the use of EDTA in all buffer solutions as this will cause degradation of the complex. See the link below to publication that may be helpful. http://diyhpl.us/~bryan/irc/protocol-online/protocol-cache/view_cache.cgi%3FID=3486

      Helpful?

  2. How to make 200 mM from 94740-250MG vanadyl ribonucleoside complexes powder?

    1 answer

    1. The method to prepare a 200mM solution of the 94740 is proprietary and involves in-process testing. However, product 94742 and R3380 are prepared 200mM solutions of Vanadyl Ribonucleoside complexes.

      Helpful?

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