76216

Papain from Carica papaya

solution, light brown, ≥10 U/mg protein (~25 mg/ml)

• Numéro CAS: 9001-73-4
• Numéro de classification (Commission des enzymes): 3.4.22.2 (BRENDA, IUBMB)
• Numéro CE : 232-627-2
• Numéro MDL: MFCD00131791
• Code UNSPSC : 12352204
• eCl@ss : 42030311
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: papaya
• Niveau de qualité: 100
• Forme: solution
• Activité spécifique: ≥10 U/mg protein (~25 mg/ml)
• Poids mol.: M_r ~23000
• Technique(s): cell culture | mammalian: suitable; single cell analysis: suitable
• Couleur: light brown
• Température de stockage: 2-8°C

Description

Description générale

Papain is a proteolytic enzyme and is naturally occurring in papaya (Carica papaya L.). It is obtained from the latex of cut unripe papaya. Papain belongs to the cysteine proteinase family. It is a globular, single-chain protein composed of four disulfide bridges and catalytically essential residues. Papain consists of an all α-domain and anti-parallel β-sheet domain.[1]

Application

Papain from Carica papaya has been used:

• in the enzymatic dissociation of rat cortices to extract astrocytes for cell culture[2]
• to study its effects on Alpaca seminal plasma viscosity and sperm function[3]
• to study its effects on the yield and cryo-survival of Rhinoceros spermatozoa[4]

Papain is used as a meat tenderizer. It′s also used to break up tissue in the preparation of primary cell cultures.

Actions biochimiques/physiologiques

Papain is a cysteine protease that cleaves peptide bonds and is known to cleave the Fc portion of antibodies.

Papain plays an important role in many biological processes in many living organisms. It exhibits proteolytic activity against proteins, amino-acid esters and amide links, and short-chain peptides. Papain preferentially cleaves the peptide bonds of basic amino acids such as arginine, phenylalanine, and lysine. It has a wide range of applications in drug discovery, pharmaceutical, food[1], baking, and dairy industries.[5]

Définition de l'unité

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-benzoyl-L-arginine ethyl ester (BAEE, Cat. No. 12880) per minute at pH 6.2 and 25°C

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H315,H319,H334,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Faceshields, Gloves