70215

2,3-Naphthalenedicarboxaldehyde

suitable for fluorescence

• Synonyme(s) : NDA
• Formule linéaire : C₁₀H₆(CHO)₂
• Numéro CAS: 7149-49-7
• Poids moléculaire : 184.19
• Numéro Beilstein : 2207267
• Numéro MDL: MFCD00016618
• Code UNSPSC : 12352108
• ID de substance PubChem : 329762349
• Nomenclature NACRES : NA.32

Propriétés

• Forme: crystals
• Impuretés: ≤2% mono- and dicarboxylic acid (¹H-NMR)
• Pf: 131-133 °C (lit.); 132-135 °C
• Fluorescence: λ_ex 420 nm; λ_em ~480 nm in 0.1 M borate pH 9.3 (after derivatization with glycine [~90 μM glycine, ~20 μM N^-])
• Adéquation: suitable for fluorescence
• Température de stockage: 2-8°C
• Chaîne SMILES: [H]C(=O)c1cc2ccccc2cc1C([H])=O
• InChI: 1S/C12H8O2/c13-7-11-5-9-3-1-2-4-10(9)6-12(11)8-14/h1-8H
• Clé InChI: ZIPLKLQPLOWLTM-UHFFFAOYSA-N

Description

Description générale

2,3-Naphthalenedicarboxaldehyde, also known as NDA, is a novel fluorescent derivatization agent. The fluorescence max. excitation of NDA is in the range of 424 nm and max. emission is achieved at 484nm.

2,3-Naphthalenedicarboxaldehyde is a fluorescent derivatization agent of primary amines, amino acids, and small peptides. The reaction between the amino compounds and NDA results in highly fluorescent and stable derivative compounds.

Application

2,3-Naphthalenedicarboxaldehyde is used as a fluorescent labelling agent for quantification of Histamine in human immunoglobulin preparations. NAD is used as a derivatization agent for glutathione to quantify protein glutathionylation. It is a suitable reagent used for the development of fluorescence-based microtiter plate assay for γ-glutamylcyclotransferase.

Caractéristiques et avantages

NDA based fluorescent assays have the following benefits over O-Phthalaldehyde (OPA):

• High Reaction rate.
• High Fluorescence quantum yield.
• No uncommon excitation wavelength.
• No side reactions.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H315,H319,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves