Sybr Green and Fluorescein sodium salt are both green dyes, and although their spectral ranges are close, it is important to consider their specific uses and characteristics. While Sybr Green is a popular dye for molecular use, Fluorescein sodium salt is a very economical product and may be useful for specific applications. However, there is no information comparing the two dyes for use in PCR.
46960
Fluorescein sodium salt
BioReagent, suitable for fluorescence
Synonyme(s) :
Acid Yellow 73, NaFl, Sodium fluorescein, Yellow No. 8, D&C;Yellow No. 8, NaFluo, Uranine
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393.00 CHF
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25 G
42.20 CHF
100 G
121.00 CHF
500 G
393.00 CHF
About This Item
Formule empirique (notation de Hill):
C20H10Na2O5
Numéro CAS:
Poids moléculaire :
376.27
Numéro C.I. (Colour Index):
45350
Beilstein:
3833041
Numéro CE :
Numéro MDL:
Code UNSPSC :
12171500
ID de substance PubChem :
Nomenclature NACRES :
NA.32
Produits recommandés
Gamme de produits
BioReagent
Niveau de qualité
Forme
powder
Technique(s)
titration: suitable
Fluorescence
λex 460 nm; λem 515 nm(lit.)
λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0
Adéquation
suitable for fluorescence
Chaîne SMILES
[Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1
InChI
1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2
Clé InChI
RGPLGPBQJOQFJS-UHFFFAOYSA-L
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Description générale
Fluorescein Sodium Salt (FSS) is a xanthene dye having a polycrystalline structure with a monoclinic system. Fluorescein is a synthetic organic compound available as a dark orange/red powder soluble in water and alcohol.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
423.7 °F - Pensky-Martens closed cup
Point d'éclair (°C)
217.6 °C - Pensky-Martens closed cup
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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For Fluorescein sodium salt 46960, is there a fluorescence value for a positive PCR sample, and how would this value translate as a concentration of this powder?
1 answer-
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