A free acid is the acidic form of a substance in which all acidic groups are fully protonated.
46955
Fluorescein
for fluorescence, free acid
Synonyme(s) :
Acid Yellow 73
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250 MG
34.30 CHF
1 G
137.00 CHF
100 G
1 230.00 CHF
500 G
2 140.00 CHF
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250 MG
34.30 CHF
1 G
137.00 CHF
100 G
1 230.00 CHF
500 G
2 140.00 CHF
About This Item
Formule empirique (notation de Hill):
C20H12O5
Numéro CAS:
Poids moléculaire :
332.31
Numéro C.I. (Colour Index):
45350
Beilstein:
94324
Numéro CE :
Numéro MDL:
Code UNSPSC :
12171500
ID de substance PubChem :
Nomenclature NACRES :
NA.32
Produits recommandés
Qualité
for fluorescence
Niveau de qualité
Technique(s)
titration: suitable
Fluorescence
λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0
Adéquation
suitable for fluorescence
Chaîne SMILES
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1
InChI
1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H
Clé InChI
GNBHRKFJIUUOQI-UHFFFAOYSA-N
Informations sur le gène
human ... PTPN1(5770) , PTPRF(5792)
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Description générale
Fluorescein dye is the most commonly used fluorescent probe.[1] It is used to stain epithelial defects and analyse the integrity of corneal epithelium. Fluorescein is used in the diagnosis of corneal perforation.[2] Oral or intravenous fluorescein is used in fluorescein angiography to detect diabetic retinopathy and choroidal neovascular membrane.[3]
Application
Fluorescein has been used:
- in the permeability assay of Caco-2 cells[4][5]
- to test its suitability to serve as lifetime fluorescence standards[6]
- as a fluorophore for the fast recovery of fluorescence peaks and fluorophore concentration in the presence of hemoglobin[7]
- as a fluorescent probe in oxygen radical absorbance capacity (ORAC) assay[8]
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Les clients ont également consulté
Absorption and fluorescence properties of fluorescein
Sjoback R, et al.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 51(6), L7-L21 (1995)
Ocular health assessment
Prokopich CL, et al.
Clinical Pediatric Nephrology, Second Edition, 221-318 (2007)
Rapid measurement of total antioxidant capacity in plants
Gillespie KM, et al.
Nature Protocols, 2(4), 867-867 (2007)
Diagnostic agents
Robert-Gnansia E
Drugs During Pregnancy and Lactation, 502-513 (2007)
Microfluidic gut-on-a-chip with three-dimensional villi structure
Shim KY, et al.
Biomedical Microdevices, 19(2), 37-37 (2017)
Articles
Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
Fluorescence quenching microscopy visualizes 2D materials like graphene and MoS2 rapidly, inexpensively, and with high fidelity.
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What is a free acid?
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What is the size in kDa of this Fluorescein?
1 answer-
This product has a molecular weight size of 332.32 g/mol or 332.32 daltons. The kDa unit designates kilodaltons, 1 kDa is equivalent to 1000 daltons.
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How long can this product be kept once it's made into a solution? If it can be kept more than 1 day; what are the storage conditions? Thank you
1 answer-
The stability of this product is not determined. However, various sources state that this product is stable in solvent at -80°C for 6 months; at -20°C for 1 month (protect from light).
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What is the dye content of Product 46955, Fluorescein?
1 answer-
According to our supplier, the dye content of pure Fluorescein is a minimum of 90%. We do not test the purity of this product since it is not one of our specifications.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How should Product 46955, Fluorescein, be stored?
1 answer-
We recommend storing this product at room temperature.
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What is Product 46955, Fluorescein, soluble in?
1 answer-
According to the chemicals encyclopedia published by the Royal Society of Chemistry : 13th Edition, Fluorescein is soluble in hot alcohol and glacial acetic acid. Also, it is soluble in alkali hydroxides or carbonates. This product is insoluble in water, benzene, chloroform and ether.
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