SCC112

FLAG-SNAP-hTERT expressing HeLa Cell Line

Human

• Code UNSPSC : 41106514
• eCl@ss : 32011203

Propriétés

• product name: FLAG-SNAP-hTERT expressing HeLa Cell Line, FLAG-SNAP-hTERT expressing HeLa Cell Line is a useful tool for studying telomerase biology and enables purification and visualization of endogenous telomerase reverse transcriptase (TERT).
• Source biologique: human
• Niveau de qualité: 100
• Conditions d'expédition: ambient

Description

Description générale

Telomerase play a critical role in regulating proliferation and senescence in normal somatic cells as well as most cancer cells. In humans, the telomerase reverse transcriptase (hTERT) is a lowly expressed protein with only several hundred molecules per cell. The low expression has made it difficult to detect, purify and study TERT activity from the endogenous loci.

To facilitate reliable detection of the endogenous TERT protein, a FLAG-SNAP tag was fused to the N-terminus of the TERT locus in HeLa cells. The FLAG-SNAP tag does not impair the function of the endogenous hTERT protein. Efficient immunopurification (IP) of the active telomerase ribonucleoprotein complexes may be accomplished by using the well-characterized FLAG antibody. The SNAP tag may be used to visualize subcellular localization of the low abundant endogenous TERT protein.

Reference:
Xi L, Schmidt JC, Zaug AJ, Ascarrunz DR, and Cech TR (2016) A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression. Genome Biology 16: 231-248.

Description de la lignée cellulaire

Cancer Cells

Application

Research Category
Cell Culture

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Qualité

• Each vial contains ≥1X10⁶ viable cells.
• Cells are tested negative for HPV-16, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
• Cells are negative for mycoplasma contamination.
• Cells are positive for HPV-18 by PCR.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable