MIT1000
Mitochondria/Cytosol Fractionation Kit
This Mitochondria/Cytosol Fractionation Kit provides reagents for quick & efficient isolation of intact mitochondria from cultured cells.
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About This Item
Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.75
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Niveau de qualité
Réactivité de l'espèce (prédite par homologie)
all
Fabricant/nom de marque
Chemicon®
Technique(s)
cell culture | mammalian: suitable
Application(s)
sample preparation
Conditions d'expédition
dry ice
Description générale
“” The synthesis of most of the adenosine triphosphate (ATP) occurs in these double membraned organelles that are found in living cells. ATP production by the mitochondria is done by the process of respiration, which uses oxygen to generate energy. This is a very efficient process for using food energy to make ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria also regulate crucial apoptosis signaling pathways. The number of mitochondria in a cell varies widely by organism and tissue type.
’ This kit allows mitochondrial isolation by using a convenient table top microcentrifuge, and can be used to separate an enriched mitochondrial fraction (heavy membrane fraction) from cytosolic fraction (light membrane fraction).
’ This kit allows mitochondrial isolation by using a convenient table top microcentrifuge, and can be used to separate an enriched mitochondrial fraction (heavy membrane fraction) from cytosolic fraction (light membrane fraction).
Application
Domaine de recherche
Apoptose et cancer
Apoptose et cancer
Composants
Isotonic Mitochondrial Buffer (CS204255):
μ
μ
μ
μ
Stockage et stabilité
°
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Sauf indication contraire dans notre catalogue ou toute autre documentation associée au(x) produit(s), nos produits sont uniquement destinés à la recherche et ne sauraient être utilisés à d'autres fins, ce qui inclut, sans s'y limiter, les utilisations commerciales non autorisées, les utilisations diagnostiques in vitro, les utilisations thérapeutiques ex vivo ou in vivo, ou tout type de consommation ou d'application chez l'être humain ou chez l'animal.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2
Code de la classe de stockage
10 - Combustible liquids
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Simon C Baker et al.
The American journal of pathology, 186(5), 1267-1277 (2016-03-24)
Recreational abuse of ketamine has been associated with the emergence of a new bladder pain syndrome, ketamine-induced cystitis, characterized by chronic inflammation and urothelial ulceration. We investigated the direct effects of ketamine on normal human urothelium maintained in organ culture
Jinxin Pan et al.
Central-European journal of immunology, 47(1), 20-29 (2022-05-24)
The protective activity of scopoletin (SPT) against glucose-induced cataract has been attributed to attenuation of aldose reductase activity and oxidative stress in a rat model. The present investigation was aimed to study the protective effect and mechanism of SPT in
Hongmei Li et al.
Nature cell biology, 15(7), 773-785 (2013-06-25)
Following exocytosis, the rate of recovery of neurotransmitter release is determined by vesicle retrieval from the plasma membrane and by recruitment of vesicles from reserve pools within the synapse, which is dependent on mitochondrial ATP. The anti-apoptotic Bcl-2 family protein
Pedro Escoll et al.
Cell host & microbe, 22(3), 302-316 (2017-09-05)
The intracellular bacteria Legionella pneumophila encodes a type IV secretion system (T4SS) that injects effector proteins into macrophages in order to establish and replicate within the Legionella-containing vacuole (LCV). Once generated, the LCV interacts with mitochondria through unclear mechanisms. We
Turki Y Alhazzazi et al.
Anticancer research, 36(1), 49-60 (2016-01-02)
The survival rate of patients with head and neck squamous cell carcinoma (HNSCC) stands at approximately 50% and this has not improved in decades. This study developed a novel sirtuin-3 (SIRT3) inhibitor (LC-0296) and examined its role in altering HNSCC
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