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790326C

Avanti

Fluorescent Transfection Reagent

Avanti Research™ - A Croda Brand 790326C

Synonyme(s) :

Fluorescent Transfection Reagent, Fluorescent Transfection Reagent, contains DOTAP:DOPE (1:1, w/w) + 0.1 wt.% NBD-DOPE

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.25

Forme

liquid

Conditionnement

pkg of 1 × 1 mL (790326C-10mg)

Fabricant/nom de marque

Avanti Research™ - A Croda Brand 790326C

Concentration

10 mg/mL (790326C-10mg)

Application(s)

advanced drug delivery

Type de lipide

cationic lipids
transfection

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Description générale

Fluorescent Transfection Reagent has the cationic lipid 1,2-dioleoyloxypropyl)-N,N,N-trimethylammonium chloride (DOTAP) and the neutral lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine in the ratio 1:1 w/w along with 0.1 wt% N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dioleoyl (NBD) DOPE.[1] NBD-DOPE is a fluorescence label.[2] NBD is attached to headgroup of DOPE.[3]

Application

Fluorescent Transfection Reagent has been used to visualize the localization of liposome oligodeoxynucleotides (ODN) complex in lung alveolar cells.[4]

Actions biochimiques/physiologiques

Fluorescent Transfection Reagent is useful in understanding fibroplex membrane structure[2] and interaction of fibroin particles with cationic lipids.[1] N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dioleoyl (NBD) is used in model membranes studies and is useful in visualizing membrane domains, at 460/535 nm (excitation/emission) wavelengths.[3] The DOTAP:DOPE with 0.1% NBD-DOPE liposome (LipNBD) reconstituted with transferrin called the transferrin liposome-nitrobenzoxadiazole (Tf-LipNBD) is useful as a probe. It provides improved image contrast in in vitro magnetic resonance imaging (MRI) of tumor cells.[5]

Conditionnement

5 mL Clear Glass Sealed Ampule (790326C-10mg)

Informations légales

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Pictogrammes

Skull and crossbonesHealth hazard
GHS06,GHS08

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Organes cibles

Central nervous system

Code de la classe de stockage

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

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Certificats d'analyse (COA)

Lot/Batch Number

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Consulter la Bibliothèque de documents

Growth hormone-dependent changes in the rat lung proteome during alveorization
Beyea JA, et al.
Molecular and Cellular Biochemistry, 321(1-2), 197-197 (2009)
Dual probe with fluorescent and magnetic properties for imaging solid tumor xenografts
Shan L, et al.
Molecular Imaging, 6(2), 7290-2007 (2007)
The influence of NBD fluorescent probe on model membranes containing POPC and DPPC
Weng CJ, et al.
Molecular Membrane Biology, 33(1-2), 23-28 (2016)
Woo-Jin Kim et al.
Biomaterials, 122, 154-162 (2017-01-26)
Directly delivering therapeutic proteins into cells has promise as an intervention without side effects for protein deficiencies caused by genetic defects. However, as negatively charged macromolecules, proteins require carriers for achieving cellular uptake and maintaining their activity in the cytoplasm.
Efficiency data of intracellular recombinant protein delivery using cationic lipid coated silk fibroin particle
Kim WJ, et al.
Data in Brief, 13, 683-691 (2017)

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