Direkt zum Inhalt
Merck

Inhibition of bovine plasma semicarbazide-sensitive amine oxidase by caffeine.

Journal of biochemical and molecular toxicology (2011-02-16)
A Olivieri, K Tipton
ZUSAMMENFASSUNG

Semicarbazide-sensitive amine oxidase (SSAO) is a copper-containing enzyme that catalyzes the oxidative deamination of endogenous and exogenous primary amines. SSAO exists in mammals both as a plasma-soluble and as a membrane-bound form, and its active site is able to come into contact with numerous xenobiotic, amine-containing compounds. The kinetic studies performed in this work showed that caffeine inhibition of bovine serum amine oxidase was noncompetitive when benzylamine was used as substrate and mixed when the substrate used was methylamine. Since caffeine contains an imidazole ring, it cannot be excluded that it might bind to an inhibitory imidazoline-binding site on SSAO.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Benzylamin, ReagentPlus®, 99%
Sigma-Aldrich
Benzylamin, purified by redistillation, ≥99.5%
Sigma-Aldrich
Benzylamin -hydrochlorid
Supelco
Benzylamin, for GC derivatization, LiChropur, ≥99.0%