821892

ToyoScreen^® Phenyl-600M Process Development Column

phase phenyl, pkg of 6 × 1 mL, 65 μm particle size

• UNSPSC-Code: 41115700

Eigenschaften

• Materialien: aluminum seal; polypropylene and polyethylene frit
• Produktlinie: ToyoScreen^®
• chemische Klasse(n) des Analyten: proteins
• Verpackung: pkg of 6 × 1 mL
• Hersteller/Markenname: Tosoh 21892
• Parameter: ≤50% organic solvent; 0.2-1 mL/min flow rate; 3 bar max. pressure; 4 mL/min max. flow rate
• Methode(n): HPLC: suitable
• L × ID: 3 cm × 6.4 mm
• Matrix: hydroxylated methacrylic polymer
• Aktive Matrixgruppe: phenyl phase
• Partikelgröße: 65 μm
• Betriebs-pH-Wert: 1-13
• Kapazität: 55 g/L±10 adsorption capacity (lysozyme)
• Trenntechnik: hydrophobic interaction (HIC)

Beschreibung

Allgemeine Beschreibung

Every large scale commercial purification begins at a small scale in a method development lab by evaluating retention and selectivity of the target protein versus impurities, host cell proteins, DNA, etc., on multiple resins, often from different vendors. Depending on the constraints, the process development engineer can either purchase bulk resins to pack several small columns with different resins or take advantage of the speed and convenience of pre-packed columns for resin evaluation. Pre-packed ToyoScreen^® Process Development Columns are available in 1 mL and 5 mL volumes packed with the most popular Toyopearl^® resins. ToyoScreen columns can be used to quickly establish optimum elution conditions. Varying pH, salt type, gradient conditions and flow rate are common experimental parameters explored. Initial results from resin screening and optimization with ToyoScreen columns accurately predict the separation behavior at larger scale.

Spezifikationen

Clean in place with 0.5 M NaOH or 0.1 M HCl.

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Rechtliche Hinweise

ToyoScreen is a registered trademark of Tosoh Corporation

Toyopearl is a registered trademark of Tosoh Corporation